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Bacillus subtilis used for degrading aflatoxins

A technology of Bacillus subtilis and aflatoxin, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of no degradation, no degradation of aflatoxin, low degradation efficiency, etc. Mild effect and high detoxification activity

Active Publication Date: 2010-05-12
河南亿万中元生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detoxification studies of some other bacteria and fungi have shown that the degradation efficiency of most bacteria is not high, and the degradation efficiency is affected by the action time, pH value of the solution, the number of bacteria, the concentration of toxins, and the concentration of metal ions. Irreversible degradation of aflatoxins in feed
[0004] So far, there is no report on the degradation of aflatoxin by Bacillus subtilis and its metabolites

Method used

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  • Bacillus subtilis used for degrading aflatoxins
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Examples

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Effect test

Embodiment 1

[0045] Embodiment 1 The cultivation method of Bacillus subtilis of the present invention

[0046] Get Bacillus subtilis Bacillus subtilis ANSB060CGMCC No.34405ml (viable bacteria concentration is 10 9 CFU / ml), inoculated in 50ml medium for shake flask fermentation culture, the fermentation temperature is 37°C, the pH value is 7.0, the rotation speed is 200r / min, and the fermentation time is 24h.

[0047] Among them, the shake flask fermentation medium is composed of the following components: peptone 10g, glucose 5g, dipotassium hydrogen phosphate 0.1g, potassium dihydrogen phosphate 1.0g, manganese sulfate monohydrate 0.5g, magnesium sulfate heptahydrate 1.0g, distilled water 1000mL ; pH value is 7.0.

[0048] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 2

[0049] The culture method of embodiment 2 Bacillus subtilis of the present invention

[0050] Get Bacillus subtilis ANSB060CGMCC No.34401ml (viable bacteria concentration is 10 9 CFU / ml), inoculated in 80ml medium for shake flask fermentation culture, the fermentation temperature is 25°C, the pH value is 7.5, the rotation speed is 225r / min, and the fermentation time is 36h.

[0051]Among them, the shake flask fermentation medium is composed of the following components: peptone 5g, glucose 8g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 2.0g, manganese sulfate monohydrate 1.0g, magnesium sulfate heptahydrate 2.0g, distilled water 1200mL ; pH value is 7.5.

[0052] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 3

[0053] Embodiment 3 The cultivation method of Bacillus subtilis of the present invention

[0054] Get Bacillus subtilis Bacillus subtilis ANSB060CGMCC No.3440 2ml (viable bacteria concentration is 10 9 CFU / ml), inoculated in 100ml medium for shake flask fermentation culture, the fermentation temperature is 45°C, the pH value is 6.5, the rotation speed is 185r / min, and the fermentation time is 18h.

[0055] Among them, the shake flask fermentation medium is composed of the following components: peptone 8g, glucose 6g, dipotassium hydrogen phosphate 0.15g, potassium dihydrogen phosphate 1.5g, manganese sulfate monohydrate 2.0g, magnesium sulfate heptahydrate 0.5g, distilled water 800mL ; pH value is 6.5.

[0056] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

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Abstract

The invention relates to a Bacillus subtilis ANSB 060CGMCC No.3440 used for degrading aflatoxins, and culture method and application thereof. The invention also provides a method for degrading aflatoxins B1, G1 and M1 by fermentation liquor of the strain. By the method, the Bacillus subtilis is reacted with the aflatoxin B1 for 2 hours, has a degradation rate of over 70 percent on the aflatoxin B1, and has the degradation rate of over 80 percent after the reaction performed for 12 hours; and the Bacillus subtilis has the degradation rates of over 80 percent and over 60 percent on the aflatoxin G1 and aflatoxin M1 respectively. The Bacillus subtilis has high detoxification activity, strong specificity, mild action effect, and no damage to nutrient in feeds, and is suitable for removing the aflatoxins in the feeds.

Description

technical field [0001] The invention relates to bacillus subtilis, in particular to a bacillus subtilis used for degrading aflatoxins B1, G1 and M1. Background technique [0002] Since the discovery of aflatoxin in 1960, scientists have continued to study the prevention and detoxification methods of this toxoid. At present, the research on aflatoxin detoxification methods mainly includes chemical treatment and physical detoxification. There are some problems in the traditional physical and chemical methods of detoxification, such as the loss of nutrition in the feed, affecting the sensory quality of the feed, and the equipment required by some methods is expensive, etc., which limits the application in actual production. [0003] Most of the reports on the use of microorganisms for detoxification have been concentrated in the past ten years. There are not many domestic studies, only Liu Daling et al. (Research on the detoxification effect of fungal extracts on aflatoxins [...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/20C12R1/125
Inventor 计成马秋刚高欣赵丽红雷元培
Owner 河南亿万中元生物技术有限公司
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