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Trichiderma longibraciatum strain

A technology of Trichoderma longbranchus and strain, applied in the field of microorganisms, can solve the problems of rare and in-depth commercial preparations

Inactive Publication Date: 2011-07-20
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is suitable for the characteristics of high and cold climates in Northeast and Northwest regions. Although there are some studies on Trichoderma that can be applied to trees and fruit trees, they are not in-depth, and commercial preparations are still rare.

Method used

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Experimental program
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Embodiment Construction

[0021]1) At first, isolate Trichoderma longibrachiatum Rifai bacterial strain T05 for testing from the litter under the 20-year-old small black poplar forest in Hongqi Forest Farm, Daqing City Forestry Bureau, Heilongjiang Province, on PDA plate medium, at 25 Cultivate the fungal strain at ℃, and it can be observed that the strain grows rapidly. The fungal strain can be cultivated by three methods, one is the plate method, that is, the conidia of the strain are diluted to 50-100 conidia under the microscope field of view of 100 times, and spread on a PDA plate, placed at 30°C, A large number of conidia can be harvested until the 5th day of cultivation; the other is a liquid culture method, that is, the conidia of the bacterial strain are adjusted to a concentration of 10 7 For cfu / mL spore suspension, add 40-80mL PDB culture solution to each 250mL Erlenmeyer flask, inoculate 1mL of spore suspension in each bottle, shake culture at 30°C and 150r / min, a large number of conidia c...

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Abstract

The invention relates to a Trichiderma longibraciatum strain, belonging to the technical field of microorganisms and named as a Trichiderma longibraciatum Rifai T05 strain, preserved in China General Microbiological Culture Collection Center on January 19th 2009 and having preservation number of CGMCC No, 2888. Conidiospore stage of the Trichiderma longibraciatum strain belongs to Deuteromycota, Hyphomycetidae, Hyphomycetales, Moniliaceae, Ascomycetes, Hypocreales and Hypocreaceae. The Trichiderma longibraciatum strain has more spore output, high efficiency, biological properties of inhibiting tree skin diseases and multiple plant diseases because of wide spectrum, and can promote the plant growth and is suitable to the industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and mainly relates to a Trichoderma longibrachiae fungus strain capable of broad-spectrum inhibiting bark diseases and plant diseases and promoting plant growth. Background technique [0002] Since the industrial revolution, especially in the last 50 years of the 20th century, the global environment has been unprecedentedly damaged and polluted, and ten global environmental problems have emerged one after another. Forestry and ecological environment construction are increasingly valued by the whole society, and the construction of ecological civilization has become an inevitable choice of human civilization. In 1992, the United Nations Conference on Environment and Development adopted the "21st Century Agenda", which established the strategic position of sustainable development in the global economy and society, thus promoting the rapid development of pollution-free agriculture and forestry and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00A01P21/00C12R1/885
Inventor 池玉杰伊洪伟
Owner NORTHEAST FORESTRY UNIVERSITY
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