Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof
A melon wilt, organic fertilizer technology, applied in the directions of organic fertilizer, organic fertilizer preparation, biological organic part treatment, etc. The ability to reproduce and function, the effect of enhanced growth and stress resistance, and high bioavailability
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[0023] (1) Isolation and identification of strains
[0024] The plants and the surrounding soil that had experienced continuous cropping obstacles and severe muskmelon wilt were collected, and the samples were stored at low temperature. Kelman's TTC Agar selective medium was used to isolate pathogenic bacteria. Then, the pathogenic bacteria of muskmelon wilt was used as an indicator bacterium to isolate antagonistic bacteria from the rhizosphere soil of healthy plants in continuous cropping muskmelon wilt disease-affected fields. Then through the pot test and the field test, the high-efficiency antagonistic strains NJY10 and NJY-IVI were finally screened out, and the genera and species were identified, and the strains were stored at -70°C in 30% glycerol. Antagonistic strain NJY10, NJY-IVI inhibition zone such as figure 1 shown.
[0025] The antagonistic bacteria NJY10 belongs to Paenibacillus polymyxa, and its main biological characteristics are: Gram stain positive (G + )...
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