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Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof

A melon wilt, organic fertilizer technology, applied in the directions of organic fertilizer, organic fertilizer preparation, biological organic part treatment, etc. The ability to reproduce and function, the effect of enhanced growth and stress resistance, and high bioavailability

Inactive Publication Date: 2010-04-07
江苏永禄肥料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] On the other hand, the incineration of rice, wheat, corn, rapeseed and other crop straws, and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste the organic fertilizer and bio-organic fertilizer products. Raw materials; a large number of nutrient resources (C, N, P, K, S and trace elements) are lost outside the soil-plant system, which obviously weakens the ability of my country's agricultural sustainable development

Method used

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  • Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof
  • Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof
  • Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof

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Embodiment Construction

[0023] (1) Isolation and identification of strains

[0024] The plants and the surrounding soil that had experienced continuous cropping obstacles and severe muskmelon wilt were collected, and the samples were stored at low temperature. Kelman's TTC Agar selective medium was used to isolate pathogenic bacteria. Then, the pathogenic bacteria of muskmelon wilt was used as an indicator bacterium to isolate antagonistic bacteria from the rhizosphere soil of healthy plants in continuous cropping muskmelon wilt disease-affected fields. Then through the pot test and the field test, the high-efficiency antagonistic strains NJY10 and NJY-IVI were finally screened out, and the genera and species were identified, and the strains were stored at -70°C in 30% glycerol. Antagonistic strain NJY10, NJY-IVI inhibition zone such as figure 1 shown.

[0025] The antagonistic bacteria NJY10 belongs to Paenibacillus polymyxa, and its main biological characteristics are: Gram stain positive (G + )...

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Abstract

The invention relates to antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer produced by the same, belonging to the technique of agricultural intensive production. In the invention, two strains of antagonistic bacteria which have remarkable antagonistic effect on the pathogenic fungi of continuous cropping melon blast disease (bacillus polymyxa NJY10 and bacillus subtilis NJY-IVI) are obtained by separation; the antagonistic bacteria is mixed with the organic fertilizer to be prepared into microorganism organic fertilizer, wherein the fertilizer comprises more than 1*108 antagonistic bacteria per gram, 4-5% of total nitrogen content, 6-10% of total nitrogen, phosphorous and potassium nutrient and 30-35% of organic matter content. Experiments show that after being fertilized into the soil, the microorganism organic fertilizer can lead the antagonistic bacteria to reproduce rapidly and form a superior group in the soil, the biological prevention and cure rate towards the melon blast disease in the continuous cropping soil with serious melon blast disease can reach over 80%; and in the soil without forming continuous cropping impediment, long-term use of the microorganism organic fertilizer of the invention can prevent and cure the occurrence of melon blast disease substantially.

Description

1. Technical field [0001] The invention relates to antagonistic bacteria capable of preventing and treating continuous-cropping muskmelon wilt and microbial organic fertilizer thereof, which belongs to the agricultural intensive production technology and is specially used for overcoming and eliminating the problem of continuous-cropping muskmelon wilt. 2. Technical background [0002] Melon is cultivated all over the world and is one of the top ten fruits in the world. It is a fresh fruit that people all over the world like to eat. The distribution range is from about 65° north latitude to about 23° south latitude, covering five continents, among which China has the largest cultivation area. It is widely planted in the north and south of my country, and Xinjiang, Gansu and other northwest regions are the traditional production areas of thin-skinned melons. Melon diseases and insect pests, especially muskmelon wilt, have always been an important problem affecting its product...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C05F11/08C05F17/00C05G3/00C05G3/04C12R1/01C12R1/125C05G3/80
CPCY02W30/40
Inventor 沈其荣赵青云徐阳春杨兴明冉伟
Owner 江苏永禄肥料有限公司
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