Traditional Chinese medicine biological agent for improving immunity of livestock and preparation method thereof
A technology for biological preparations and immunity, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of disproportionate development of animal husbandry, low bioavailability, and low technical content, and improve livestock and poultry. Immunity and production performance, low bioavailability, dose reduction effect
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Embodiment 1
[0028] The preparation of embodiment 1 Chinese medicine biological preparation of the present invention
[0029] Composition of traditional Chinese medicine: 100 parts of Eucommia leaves, 100 parts of Grifola frondosa, 50 parts of Astragalus membranaceus, 50 parts of Epimedium. Preparation method:
[0030] (1) Inoculate the bacillus into the common broth medium, 37 ℃, 220rpm shaker culture to produce the first-grade seed liquid, when the viable bacteria content reaches 1.0×10 8~9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 10%, the cultivation temperature is 30°C, the stirring speed is 220rpm, and the live bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop culturing and reserve;
[0031] (2) Get the Chinese medicinal material of formula quantity, be ground into 120 purpose medicinal material fine powders, pack in fermenter, add and account for 20% bran of Chinese medicine weig...
Embodiment 2
[0035] Embodiment 2 The preparation of Chinese medicine biological preparation of the present invention
[0036] Composition of traditional Chinese medicine: 70 parts of Eucommia leaves, 60 parts of Grifola frondosa, 40 parts of Astragalus membranaceus, 30 parts of Epimedium. Preparation method:
[0037] (1) Inoculate the bacillus into the common broth medium, 36°C, 230rpm shaker culture to produce the first-grade seed liquid, when the viable bacteria content reaches 1.0×10 8~9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 8%, the culture temperature is 29°C, the stirring speed is 230rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop culturing and reserve;
[0038] (2) Get the Chinese herbal medicine of formula quantity, be ground into 100 purpose medicinal material fine powders, pack in fermenter, add and account for the weight of traditional Chinese medicin...
Embodiment 3
[0041] Embodiment 3 The preparation of Chinese medicine biological preparation of the present invention
[0042] Composition of traditional Chinese medicine: 125 parts of Eucommia leaves, 125 parts of Grifola frondosa, 80 parts of Astragalus membranaceus, 70 parts of Epimedium. Preparation method:
[0043] (1) Inoculate bacillus into common broth medium, 35°C, 200rpm shaker culture to produce first-grade seed liquid, when the viable bacteria content reaches 1.0×10 8~9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 9%, the cultivation temperature is 30°C, the stirring speed is 200rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop culturing and reserve;
[0044] (2) Get the Chinese medicinal material of formula quantity, be ground into 90 purpose medicinal material fine powders, pack in fermenter, add and account for the weight of traditional Chinese medicine 25...
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