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Traditional Chinese medicine biological agent for improving immunity of livestock and preparation method thereof

A technology for biological preparations and immunity, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of disproportionate development of animal husbandry, low bioavailability, and low technical content, and improve livestock and poultry. Immunity and production performance, low bioavailability, dose reduction effect

Active Publication Date: 2010-04-07
BEIJING DABEINONG ANIMAL HEALTH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Chinese herbal immune enhancers currently used are generally powders with low technical content, which are directly added to the feed for use, with low bioavailability and poor palatability, which is extremely disproportionate to the development of animal husbandry.

Method used

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  • Traditional Chinese medicine biological agent for improving immunity of livestock and preparation method thereof
  • Traditional Chinese medicine biological agent for improving immunity of livestock and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 Chinese medicine biological preparation of the present invention

[0029] Composition of traditional Chinese medicine: 100 parts of Eucommia leaves, 100 parts of Grifola frondosa, 50 parts of Astragalus membranaceus, 50 parts of Epimedium. Preparation method:

[0030] (1) Inoculate the bacillus into the common broth medium, 37 ℃, 220rpm shaker culture to produce the first-grade seed liquid, when the viable bacteria content reaches 1.0×10 8~9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 10%, the cultivation temperature is 30°C, the stirring speed is 220rpm, and the live bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop culturing and reserve;

[0031] (2) Get the Chinese medicinal material of formula quantity, be ground into 120 purpose medicinal material fine powders, pack in fermenter, add and account for 20% bran of Chinese medicine weig...

Embodiment 2

[0035] Embodiment 2 The preparation of Chinese medicine biological preparation of the present invention

[0036] Composition of traditional Chinese medicine: 70 parts of Eucommia leaves, 60 parts of Grifola frondosa, 40 parts of Astragalus membranaceus, 30 parts of Epimedium. Preparation method:

[0037] (1) Inoculate the bacillus into the common broth medium, 36°C, 230rpm shaker culture to produce the first-grade seed liquid, when the viable bacteria content reaches 1.0×10 8~9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 8%, the culture temperature is 29°C, the stirring speed is 230rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop culturing and reserve;

[0038] (2) Get the Chinese herbal medicine of formula quantity, be ground into 100 purpose medicinal material fine powders, pack in fermenter, add and account for the weight of traditional Chinese medicin...

Embodiment 3

[0041] Embodiment 3 The preparation of Chinese medicine biological preparation of the present invention

[0042] Composition of traditional Chinese medicine: 125 parts of Eucommia leaves, 125 parts of Grifola frondosa, 80 parts of Astragalus membranaceus, 70 parts of Epimedium. Preparation method:

[0043] (1) Inoculate bacillus into common broth medium, 35°C, 200rpm shaker culture to produce first-grade seed liquid, when the viable bacteria content reaches 1.0×10 8~9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 9%, the cultivation temperature is 30°C, the stirring speed is 200rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop culturing and reserve;

[0044] (2) Get the Chinese medicinal material of formula quantity, be ground into 90 purpose medicinal material fine powders, pack in fermenter, add and account for the weight of traditional Chinese medicine 25...

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Abstract

The invention relates to a traditional Chinese medicine biological agent for improving immunity of livestock and a preparation method thereof; the traditional Chinese medicine biological agent is prepared by combining traditional Chinese medicine formulas and modern microbiological technology; the biological agent adopts folium cortex eucommiae, grifola frondosa, astragalus root and herba epimedii, which belong to traditional Chinese medicines, as raw materials; bran, bean pulp, ammonium sulfate, magnesium sulfate, calcium carbonate and water are added in the raw materials after the raw materials are crushed, so as to form fermentation medium, strain nutrient solution with a certain proportion is added in the fermentation medium for fermentation, the mature solid fermentation broth is added in alcohol for extraction, separation, filtration, concentration, so as to prepare various dosage forms. The traditional Chinese medicine biological agent prepared by microbial fermentation metabolism can remarkably improve the immunity of livestock organisms, enhance the immunity of the organisms, improves the production performance of the livestock and effectively prevent immunity inhibitive diseases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry and a preparation method thereof, belonging to the field of veterinary medicine. Background technique [0002] In recent years, in the production of livestock and poultry, it is common that the preventive effect of vaccination is not good, and the dosage of the drug is gradually increased, but the ideal therapeutic and preventive effect is still not achieved. This is due to a large number of abused drugs and unreasonable vaccines for a long time Inoculation leads to a decline in the immune function of livestock and poultry, especially in a depressed or suppressed state of immune cells such as lymphocytes and macrophages; in addition, new animal diseases have emerged in recent years and pathogens of old diseases have mutated and virulently increased. To solve this problem, it is necessary to take effective measures to activa...

Claims

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Application Information

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IPC IPC(8): A61K36/539A61P37/04A23K1/00C12P1/04C12R1/10C12R1/125A23K10/12A23K10/30
Inventor 黄建茹杨秀玉莫云刘钧刘澜葛冰张俊儒葛代兴田玲邓利斌丛日刚
Owner BEIJING DABEINONG ANIMAL HEALTH TECH
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