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Method and device for detecting organic phosphorus pesticide

A technology for organophosphorus pesticides and detection methods, which is applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, which can solve problems such as cumbersome operations, and achieve the effects of simple preparation, improved sensitivity, and easy automation

Active Publication Date: 2010-03-31
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the actual measurement process, it is necessary to manually add the cholinesterase substrate, and the operation is cumbersome.

Method used

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  • Method and device for detecting organic phosphorus pesticide
  • Method and device for detecting organic phosphorus pesticide
  • Method and device for detecting organic phosphorus pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take the organophosphorus pesticide-parathion in water tested by this electrode as an example. Its determination steps are as follows:

[0029] a. Use the ion-selective electrode as the working electrode, the saturated calomel electrode as the reference electrode, and the PXSJ-216L ion meter to measure the potential value; the ion-selective electrode 4 and the saturated calomel electrode 5 are connected to the PXSJ-216L ion meter (see figure 2 ). Insert the ion selectively into the measuring cell filled with 0.03U / mL butyrylcholinesterase, use the butyrylcholine exuded from the inner filling liquid during the electrode detection as the substrate, and record the potential value at eight minutes, Measure the potential value -130.1mV, and record the electrode potential value as a control signal. The ion selective electrode 4 (see figure 1 ) is inserted with an Ag-AgCl internal reference electrode 1, and at the same time inject 0.01mol / L butyrylcholine filling solution ...

Embodiment 2

[0037] Firstly, tap water was taken to configure two spiked samples, the concentration of which was 0.5ng / ml and 2ng / ml, and the Li potential difference was measured according to the above steps b and c, compared with the standard working curve (such as Figure 6 ) calculates corresponding concentration, and the recovery rate of standard addition of mensuration is respectively 101% and 98%; Wherein inner filling liquid butyrylcholine is 10 -6 mol / L.

Embodiment 3

[0039] a. The ion selective electrode is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the PXSJ-216L ion meter is used to measure the potential value. Insert the ion-selective electrode into the measuring cell filled with 10mLpH=7.4 0.02mol / L phosphate buffer solution, use the inner filling liquid seeped out during the electrode detection as the substrate, and measure the potential at eight minutes after the electrode has calmed down. The potential value is -122.3mV; the filling liquid butyrylcholine is 10 -4 mol / L.

[0040] b. Insert the ion-selective electrode into the measuring cell filled with 10mL pH=7.4 0.02mol / L phosphate buffer solution and 0.06U / mL butyrylcholinesterase quasi-solution, and use the inner filling liquid that seeps out when the electrode detects As a substrate, the potential generated after being stabilized for eight minutes is -133.7mV. The electrode produces different potential drops at differen...

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Abstract

The invention relates to detection of organic phosphorus pesticide, in particular to a method and a device for detecting organic phosphorus pesticide. The method comprises the following concrete steps: inserting an ion selective electrode into a measuring pool and adopting seeped inner filling liquid during electrode detection as a substrate to generate a contrast signal; inserting the electrode into the measuring pool containing butyrylcholine esterase and standard pesticide and adopting the seeped inner filling liquid during electrode detection as the substrate to generate a standard signal; obtaining a standard curve by a potential difference of the standard signal and the contrast signal; finally inserting the electrode into the measuring pool containing butyrylcholine esterase and a sample to be detected and adopting the seeped inner filling liquid during electrode detection as the substrate to generate a sample signal; and obtaining the concentration of the organic phosphorus pesticide to be detected by the potential difference. The device adopts the structure that butyrylcholine inner filling liquid is contained in the electrode, an internal reference ratio electrode is inserted into the butyrylcholine inner filling liquid, and the bottom of the electrode is stuck with a polymer sensitive film. The electrode inner filling liquid can continuously seep, the enzyme substrate is generated at the original site of the surface of the electrode without manually adding the substrate, the operation is simple and convenient, and an error is prevented.

Description

technical field [0001] The invention relates to the detection of organophosphorus pesticides, in particular to a method for detecting organophosphorus pesticides and a device thereof. Background technique [0002] According to the chemical characteristics and toxicological properties of organophosphorus pesticides, there are three main analytical methods for the detection of organophosphorus pesticides, namely spectroscopy, chromatography and enzyme inhibition. The spectroscopic method requires a special chromogenic reagent, and the sensitivity is not high. There are many interference factors in the test, and false negatives are prone to occur. Chromatography has defects such as slow monitoring speed, poor continuity, high analysis cost, secondary pollution and inability to monitor online. The biggest advantage of the enzyme inhibition method is that it is easy to operate, fast, and does not require expensive instruments. It is especially suitable for on-site detection and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/49G01N27/333
Inventor 秦伟丁家旺
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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