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Trichosporon montevideense and application thereof in degradation of phenol

A technology of filamentous yeast and filamentous spore is applied in the field of microorganisms, which can solve the problems of environmental threats and achieve the effect of rapid degradation

Inactive Publication Date: 2010-03-03
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As a petrochemical derivative product and one of the main chemical raw materials, phenol poses a threat to the environment when it exists in the environment in the form of waste, and the activated sludge method has some limiting factors for the treatment of phenol wastewater, because phenol has protoplast toxi

Method used

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  • Trichosporon montevideense and application thereof in degradation of phenol
  • Trichosporon montevideense and application thereof in degradation of phenol
  • Trichosporon montevideense and application thereof in degradation of phenol

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1 Strain seed culture medium was cultured overnight, and then 10ml of bacterial liquid was centrifuged and washed with water. It was connected to 20ml of artificial phenol wastewater with different concentrations. The culture was aerated at 30°C, and the degradation results after 24hrs were as follows image 3 Shown.

[0039] Its phenol resistance concentration reaches 2500mg / L (such as image 3 ) Shows that the degradation rate of 1200mg / L of phenol reaches more than 99% within 24h, and the residual concentration of the strain after degrading phenol can be lower than 0.5mg / L, which meets the emission standards.

Example Embodiment

[0040] Example 2 Strain seed culture medium was cultured overnight, and then 10ml was centrifuged and washed with water. It was connected to 20ml artificial wastewater containing 2.5mmol / L of different heavy metals. The culture was aerated at 30°C, and the degradation results after 24hrs were as follows Figure 4 Shown.

[0041] When the concentration of heavy metal manganese, lead, copper, zinc ions is 2.5mmol / L, the degradation rate can still be as high as 98%, which has little effect on the degradation of phenol by this strain.

[0042] Nanjing Normal University

[0043] A Trichosporium Strain and Its Application in Degrading Phenol

[0044]

[0045] 1

[0046] PatentIn version 3.3

[0047] 1

[0048] 611

[0049] DNA

[0050] Trichosporon montevideense

[0051] 1

[0052] tacaggcatt cccctagtac ggcgagtgaa ccgggaaaag ctcaaatttg aaatctggct 60

[0053] gtcttcgata gtccgagttg taatctatag aagcgttttc cgtgctgaac tgtgtctaag 120

[0054] tcccttggaa cagggtatca aagagggtga taatcccgta cttgacacaa ...

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Abstract

The invention discloses a trichosporon montevideense strain used for effectively degrading phenol, aiming at providing a phenol-degrading bacterium and application of utilizing the phenol-degrading bacterium to degrade the phenol. The phenol-degrading bacterium provided by the invention is trichosporon montevideense with the collection number of CGMCC No.3144. A method provided by the invention comprises: biological degradation is carried out on phenol sewage by utilizing the trichosporon montevideense with the collection number of CGMCC No.3144. The process comprises: the phenol is degraded by switching the sewage through slant seed culture and at least two levels of seed culture or activated sludge taking the trichosporon montevideense with the collection number of CGMCC No.3144 as dominant bacteria. The technology of the invention has the advantages of tolerating high concentration phenol and heavy metal toxicity, rapidly and effectively eliminating phenol pollution, etc.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of phenol-degrading bacteria and its application in environmental pollution control. Background technique [0002] Phenol is a common raw material for organic synthesis. Phenolic compounds are widely found in coking, pharmaceutical, papermaking, dyestuff, phenolic resin synthesis, and petrochemical industries. Phenol is the main pollutant in wastewater containing phenol. According to the statistics of the State Environmental Protection Agency in 2003 The emission of phenolic substances in my country is about 3,000 tons. Phenol belongs to protoplasmic poison, has toxic effect to organisms, and is especially serious to the harm of human nervous system (Paula M et al., Isolation and characterization of phenol-degrading denitrifying bacteria [J]. Appl. Environ. Microbiol., 1998, 64 (7 ): 2432-2438.), when the phenolic compound content in the water reaches 0.005mg / L, it cannot be ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C02F3/34C12R1/645C02F101/34
Inventor 刘惠
Owner NANJING NORMAL UNIVERSITY
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