Method of female sterile gene FST for hybrid rice breeding
A technology for hybrid rice and male sterile lines, applied in the field of hybrid rice breeding with the female sterile gene FST, can solve the problems of mixed maintainer and restorer lines, avoid the cost of impurity removal, accelerate the process of popularization and application, and eliminate mechanical mixed effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0014] Seed production system (MS+FS) of the present invention, method is as follows:
[0015] 1) Propagate the rice male sterile line Heline 42A according to the current three-line method;
[0016] 2) Propagate the female sterile line Ansanbyeo / G39 / / W1944 (female sterile line, AGW-fsfs for short) by means of perennial root asexual reproduction;
[0017] 3) The rice male sterile line and the female sterile line are planted in the same field, the planting specification is 42A: AGW-fsfs=12:2, no impurities are removed during the planting period, artificial pollination is carried out to assist pollination during flowering, and the rest The cultivation management method is the same as that of ordinary seed production; during seed production, the female parent, the pollen recipient, is the male sterile line, the male parent, the pollen donor, is the female sterile line (FS), and the female sterile line pollens the male sterile line, Harvest seeds as hybrids;
[0018] 4) When matu...
Embodiment 2
[0024] Seed production system (MS+FS) of the present invention, method is as follows:
[0025] 1) Propagate the rice male sterile line Heline 42A according to the current three-line method;
[0026] 2) Cultivate the transgenic female sterile line Ansanbyeo / G39 (conditional expression dual-purpose female sterile line, referred to as AG-fsfs, genotype is RfRf-fsfs-CIP::FST), according to the chemical reagents required for promoter expression, During the flowering period, artificially spray the florigen, regulate the expression of FST gene to restore the female fertility, and complete the self-reproduction of the female sterile line;
[0027] 3) Mix the seeds of 42A and AG-fsfs (conditional expression dual-purpose type) according to the ratio of 6:1, mix and sow the mixed seeds according to the conventional seedling raising and planting specifications, do not remove impurities during planting, and do not artificially perform flowering Pollination is assisted by pollination, and ...
Embodiment 3
[0034] Seed production system (MS+FS) of the present invention, method is as follows:
[0035] 1) Propagate the rice male sterile line Diantype 1A according to the current two-line method;
[0036]2) Propagate the female sterile line Nan 29 / G39 / / W1944 (female sterile line, NGW-fsfs for short) by means of perennial root asexual reproduction;
[0037] 3) Plant the male sterile line and the female sterile line of rice in the same field, the planting specification is Dian type 1 No. A:NGW-fsfs=12:2, do not remove impurities during planting, and artificially catch pollination to assist pollination during flowering , and the rest of the cultivation and management methods are the same as ordinary seed production; when producing seeds, the female parent, the pollen recipient, is the male sterile line, the male parent, the pollen donor, is the female sterile line (FS), and the female sterile line pollens the male sterile line line, the harvested seeds are hybrids;
[0038] 4) When ma...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com