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Vitro rapid propagation method of kefir lily by using seedling ovaries as explant

A technology of explants and Clivia, which is applied in the field of plant propagation, can solve the problems of limiting the application of Clivia tissue culture technology, and achieve the effects of low cost, good growth status and the same genetic background

Inactive Publication Date: 2010-02-03
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This limits the application of Clivia tissue culture technology in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Explant inoculation and callus induction: get the young inflorescence of Clivia grandiflora, put it into a sterile ultra-clean bench, soak it for 30 seconds with 75% (volume / volume) alcohol, and use 0.1% (mass / volume) liter of Soak in mercury solution for 3 minutes, rinse with sterile water 3 times. The young inflorescence ovary was cut off, cut longitudinally twice from the middle, and inoculated in a mixture containing 6-BA1.0mg / L, NAA1.0mg / L, 2,4-D1.0mg / L, sucrose 3.5% (mass / volume) Cultured on MS medium at 20°C under scattered light, subcultured once every 30 days, and induced differentiation when yellow and compact callus grew.

[0013] 2. Induction of adventitious buds: transfer the yellow callus to the induction differentiation medium: MS+6-BA0.5mg / L+NAA0.5mg / L+3.5% sucrose, subculture once every 30 days, and light every day at 20°C 8 hours. The callus differentiation ability can last for more than one year, and more than 100 regenerated shoots can be differ...

Embodiment 2

[0017] 1. Explant inoculation and callus induction: get the young inflorescence of Clivia grandiflora, put it into a sterile ultra-clean bench, soak it for 60 seconds with 75% (volume / volume) alcohol, and use 0.2% (mass / volume) liter of Soak in mercury solution for 10 minutes, rinse with sterile water 6 times. The young inflorescence ovary was excised, cut longitudinally twice from the middle, and inoculated in a mixture containing 6-BA1.0mg / L, NAA1.0mg / L, 2,4-D1.0mg / L, sucrose 3% (mass / volume) On MS medium, cultured under scattered light at 28°C, subcultured once every 50 days, and induced differentiation when yellow dense callus grew.

[0018] 2. Induction of adventitious buds: transfer the yellow callus to the induction differentiation medium: MS+6-BA 1mg / L+NAA 4mg / L+3% sucrose, subculture once every 50 days, and light for 10 hours a day at 28°C . The callus differentiation ability can last for more than one year, and more than 100 regenerated shoots can be differentiated...

Embodiment 3

[0022] 1. Explant inoculation and callus induction: get the young inflorescence of Clivia grandiflora, put it into a sterile ultra-clean bench, soak it for 40 seconds with 75% (volume / volume) alcohol, and use 0.15% (mass / volume) liter of Soak in mercury solution for 7 minutes, rinse with sterile water 4 times. The young inflorescence ovary was excised, cut longitudinally twice from the middle, and inoculated in a medium containing 6-BA1.0mg / L, NAA1.0mg / L, 2,4-D1.0mg / L, sucrose 3.2% (mass / volume) On MS medium, cultured under scattered light at 25°C, subcultured once every 40 days, and induced differentiation when yellow dense callus grew.

[0023] 2. Induction of adventitious buds: transfer the yellow callus to the induction differentiation medium: MS+6-BA 0.75mg / L+NAA2mg / L+3.2% sucrose, subculture once every 40 days, and light for 9 hours a day at 25°C . The callus differentiation ability can last for more than one year, and more than 100 regenerated shoots can be differenti...

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Abstract

The invention belongs to a plant propagation method, particularly relating to an asexual rapid propagation method of flowers. In the method, technologies of explant inoculation, callus induction, adventitious bud induction, root induction, regeneration plant transplantation and used, ovary longitudinal cutting blocks of seedling inflorescence of clivia miniata are used as explants, so that kefir lilies can generate large quantities of regeneration plants having the entirely same gene background and unified plant type within short time. The dedifferentiation ratio can reach more than 80%, and the differentiation ratio can reach 70%. Calluses induced by one explant can be differentiated into more than 100 cluster buds through repeated regeneration; the rooting rate of the cluster buds can reach 100%, and the survival rate of transplantation in favorable environment can reach 100%; after transplantation, plants can grow favorably, and the cost of each regeneration plant is low. The methodis suitable for the rapid propagation of good and scarce species of kaffir lilies.

Description

technical field [0001] The invention belongs to a plant propagation method, in particular to an asexual rapid propagation method of flowers. Background technique [0002] Plant tissue culture is plant aseptic culture technology, which is based on the totipotency of plant cells, using isolated plant organs (such as roots, stems, leaves, stem tips, flowers, fruits, etc.), tissues (such as cambium, epidermis, Cortex, endosperm, etc.) or cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts can induce callus, adventitious buds under sterile and suitable artificial medium and artificial conditions such as light and temperature. , adventitious roots, forming whole plants or techniques for producing other products of economic value. The regenerated plants produced by plant tissue culture can basically maintain the excellent traits of the parents (explant source plants) except for extremely low variation. Since the invention of plant tissue culture technolog...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王丽王钦美
Owner NORTHEAST NORMAL UNIVERSITY
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