ELISA kit for detecting porcine circovirus antibody II

Abstract

The invention relates to an ELISA kit for detecting porcine circovirus antibodies II, wherein, the kit is developed according to the double antigen sandwich ELISA principle. The ELISA kit comprises a prepacked ELISA antigen plate bar, a cleaning solution, 100 times concentrated enzyme labeled antigen, an enzyme labeled dilution, a zymolyte coloration, a stopping solution, standard PCV2 negative sera, and standard PCV2 positive sera. The kit replaces enzyme labeled anti-antibodies with the enzyme labeled antigen. The enzyme labeled antigen can not combine with antibodies absorbed with ELSA plate without specificity. Meanwhile, the serum specimen to be detected does not need to be pre-diluted, and the serum specimen can be directly mixed with the enzyme labeled antigen with working concentration to be directly used for determination. The ELISA kit has the advantages of simple operation and shorter detecting time.

Description

ELISA kit for detection of porcine circovirus type 2 antibody Technical field: The invention relates to an ELISA kit for detecting porcine circovirus type 2 (PCV2) antibody, belonging to the technical field of veterinary biology. It is used for the antibody detection of porcine circovirus 2, so as to judge the infection situation of porcine circovirus type 2 in pig herds and monitor the prevalence of the disease and the immune effect. Background technique: Porcine circovirus type 2 (porcine circovirus 2, PCV2) is closely related to many diseases that occur in pigs, such as weaned piglet multisystem wasting syndrome (Postweaning multisystem wasting syndrome, PMWS) porcine dermatitis and nephropathy syndrome (PDNS), sow reproductive disorders , proliferative enteritis, piglet congenital tremor, porcine respiratory disease syndrome, etc. We collectively refer to various diseases caused by PCV2 as PCVD (porcine circovirus disease, PCVD). Among many PCVDs, PMWS is the most comm...

AI Technical Summary

Problems solved by technology

The ELISA method currently used to check PCV2 antibodies is mainly an indirect method. The disadvantage of this method is that the high concentration of non-specific antibodies contained in the serum can be directly adsorbed on the solid phase carrier, and sometimes also on the surface of the coated antigen. , resulting in high background or possible false positive results

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