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Manganese ion diagnosis/determination kit and method for determining manganese ion concentration

A manganese ion, kit technology, applied in the field of medical/food/environmental testing and determination

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The manganese ion diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0037] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0038] Stabilizer 500mmol / L

[0039] Coenzyme 3mmol / L

[0040] Oxalate oxidase 10000U / L

[0041] NAD(P)H oxidase 12000U / L

[0042] Oxalic acid 12mmol / L

[0043] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0044] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of manganese ion sample to reagent is 1 / 25, The reaction direction is positive reaction (rising reaction), the delay time is about 1 minute, and the detection time is about 2 minutes.

[0045] After adding the samples and reagents, let them mix and react, and...

Embodiment 2

[0047] The manganese ion diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0048] Reagent 1

[0049] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0050] Stabilizer 50mmol / L

[0051] Coenzyme 3mmol / L

[0052] Oxalic acid 12mmol / L

[0053] Reagent 2

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Oxalate oxidase 10000U / L

[0057] NAD(P)H oxidase 12000U / L

[0058] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0059] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the manganese ion sample to be tested and reagent 1, reagent 2 is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the de...

Embodiment 3

[0062] The manganese ion diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0063] Reagent 1

[0064] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0065]Stabilizer 50mmol / L

[0066] Coenzyme 3mmol / L

[0067] Oxalic acid 12mmol / L

[0068] Reagent 2

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 500mmol / L

[0071] NAD(P)H oxidase 12000U / L

[0072] Reagent 3

[0073] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 500mmol / L

[0075] Oxalate oxidase 10000U / L

[0076] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0077] When measuring manganese ion concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405...

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PUM

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Abstract

The invention relates to a manganese ion diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining manganese ion concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food and environment. The kit comprises the following main components: buffer solution, coenzyme, oxalic acid, oxalate oxidase, NAD(P)H oxidase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the concentration of manganese ion.

Description

Technical field [0001] The invention relates to a manganese ion diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of manganese ions, which belongs to the technical field of medical / food / environmental inspection and measurement. Background technique [0002] As a trace element, manganese is an indispensable and essential component in the human body, and it is also a toxic substance to the human body. In the natural state, it exists in bivalent, trivalent and tetravalent forms. The lower the degree of oxidation, the higher the toxicity. Typically manganese has eight different oxidation states. The biological significance of manganese is that it has a variety of functions as a cofactor in the human body. However, in many enzyme activation reactions, Mn 2+ and Mg 2+ Can be substituted for each other. [0003] Graphite furnace atomic absorption spectrophotometry and atomic emission ICP-OES are commonly use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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