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Method for regenerating and quickly propagating Cephalotaxus fortunei plant

A technology of Chinese fir and plant, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve the problems of short multiplication time, low seedling emergence rate, high survival rate, etc., and achieve the effect of good growth and significant economic benefits

Inactive Publication Date: 2010-01-06
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a tissue culture method for plant regeneration and rapid propagation of Cedarwood, which solves the problem of long dormancy time, long seedling time and low emergence rate of Cedarwood seeds in the prior art, so it is difficult to obtain an ideal germination rate. And more healthy seedlings, the buds cultivated by this method are healthy, strong, short in proliferation time, high in survival rate after transplanting into seedlings, and the seedlings grow robustly after survival, and their growth is good, which has significant economic benefits

Method used

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  • Method for regenerating and quickly propagating Cephalotaxus fortunei plant
  • Method for regenerating and quickly propagating Cephalotaxus fortunei plant

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Embodiment

[0019] Material collection method: select the stem segment that has germinated full new buds in that year, cut it at a distance of 1-2 cm from the bud point, remove excess leaves, and wrap it with a damp cloth after picking.

[0020] Medium:

[0021] MS basic basic medium was prepared with the formula in "Wood Plant Tissue Culture and Its Application" published by Beijing Higher Education Press and edited by Chen Zhenghua in 1986.

[0022] The preparation of the medium: the basic medium is MS, the growth hormone is IBA, NAA, IAA, ZE (zeatin), KT (phytohormone), and the Su (sucrose) is 30g / L in the medium, Ag + 7.5g / L, PH value is 5.8, the additive is activated carbon Ac; the thickness of the medium is generally 1.4 ~ 1.6cm;

[0023] Bud induction medium→MS+ZE(3.0mg / L)+KT(1.0mg / L)+IAA(1.0mg / L)+NAA(0.3mg / L)+Ag + (7.5g / L)+Su(30g / L)+Ac(2~2.5g / L)

[0024] Bud Proliferation Medium→MS+ZE(2.0mg / L)+KT(2.0mg / L)+NAA(0.5mg / L)+Ag + (7.5g / L)+Su(30g / L)+Ac(2~2.5g / L)

[0025] Rooting medi...

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Abstract

The invention provides a method for culturing in vitro of Cephalotaxus fortunei and regenerating a plant, which belongs to a culture technology and solves the problems that ideal germination percentage and more healthy seedlings are difficult to obtain due to long dormancy time of seeds, long time of seedlings and low emergence rate. The method comprises the following steps: selecting stem sections of sprouting full sprouts during that time; sprouting new healthy sprouts by inducement culture for 15-20 days; propagating the new sprouts for 25-30 days; inducing the sprouts for 20-25 days to take root and form seedlings and transplant with the survival rate over 95 percent. The propagation coefficient is higher, and nursery stock survives and has favorable growth vigor.

Description

technical field [0001] The invention belongs to the cultivation technology of Cedarwood, and more specifically relates to a method for culturing isolated embryos of Cedarcus and plant regeneration. Background technique [0002] It is an ancient relic plant and is a unique and rare tree species in my country. There are 9 species in total. There are 8 species in China, including 3 species in Fujian Province. Its leaves resemble fir, but are soft and not prickly. The winter buds at the ends of the branches are arranged in a triangle. The tree is graceful, dignified and beautiful, with peculiar shape. There are two silver-white stomatal belts on the back of the leaves. When the breeze blows, the silver light is dazzling and has a unique style. Three-pointed fir wood is solid, with straight grain and compact structure, suitable for high-end furniture and interior decoration. Its seeds have a high oil yield and can be used in industry. In addition, the roots, stems, leaves, bar...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何碧珠何官榕
Owner FUJIAN AGRI & FORESTRY UNIV
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