Method for washing and recycling membranate glass slide for microdissection

A technology of microdissection and glass slides, which is applied in the direction of cleaning methods using tools, cleaning methods and utensils, and cleaning methods using liquids. Scientific research funding, low price, and quantity saving effect

Active Publication Date: 2009-11-11
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology solves problems by protecting both the membranes from harsh conditions while also being able to effectively remove any impurities or contaminants left behind when treatments have been completed. It uses two different solutions - one contains an alkaline substance called ammonia water (AOH) and another containing hydrogen peroxide-water mixture instead of just pure nitrogen gas for better results. Overall, this method provides technical benefits over existing methods without damaging them at very high temperatures and under extreme pH levels.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the efficiency at separating cellular or histological samples from their original substrate through LSCM techniques while also ensuring consistently good results during various experiments conducted throughout different laboratories around the globe.

Method used

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  • Method for washing and recycling membranate glass slide for microdissection

Examples

Experimental program
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Effect test

Embodiment 1

[0030] This method is used to clean the microdissected membranous slides of residual lung cancer tissue (paraffin-embedded specimens).

[0031] Take two microdissected membranous glass slides pasted with lung cancer tissue (paraffin-embedded specimen), one of which is not cleaned by this method, and one is cleaned by this method.

[0032] Place the microdissected membrane slides in xylene and incubate at 50°C for 20 minutes (this step is not required for frozen tissue specimens); place the slides in a mixed solution of boric acid, alcohol, and EDTA (mixed solution) A is a mixed solution of 1% boric acid, 70% alcohol, and 0.25mol / L EDTA, with a volume ratio of 1:98:1) soak in, shake for 4 minutes, and repeat; rinse with double distilled water for 2 minutes; Transfer the sexual slides to a pH 6, 80ug / ml proteinase K solution, and incubate at 56°C for 2 hours; place the slides in clean water, touch the specimens on the membrane with fingers with plastic gloves, and rinse with double ...

Embodiment 2

[0034] The cleaning and reuse method of microdissected membrane slides can be implemented in sequence as follows:

[0035] (1) Place the microdissected membrane slides in xylene and incubate them in an incubator at 45~50℃ for 20~30 minutes;

[0036] (2) Immerse the membrane glass slide in the mixed solution A of boric acid, alcohol and EDTA, shake for 3 minutes, and repeat; the concentration of boric acid in the mixed solution A is 1% and the concentration of alcohol is 70%. The concentration of EDTA is 0.25 mol / L; the volume ratio of boric acid, alcohol and EDTA is 0.5:96.5:0.5.

[0037] (3) Rinse with double distilled water for 3 minutes; transfer the membrane glass slide to proteinase K solution and incubate at 56°C for 1 hour; the pH of proteinase K solution is 6, and the concentration is 90ug / ml.

[0038] (4) Put the glass slide in clean water, touch the specimen on the membrane of the membranous glass slide with a finger with a plastic glove, and rinse with double distilled ...

Embodiment 3

[0042] The cleaning and reuse method of microdissected membrane slides can be implemented in sequence as follows:

[0043] (1) Soak the membrane slide in the mixed solution A of boric acid, alcohol and EDTA, shake for 5 minutes, and repeat; the concentration of the boric acid is 1%; the concentration of the alcohol is 70%, the The concentration of EDTA is 0.25 mol / L, and the volume ratio of boric acid, alcohol and EDTA is 1:97:1.

[0044] (2) Rinse with double distilled water for 3 minutes; transfer the membrane slide to the proteinase K solution and incubate at 56°C for 2 hours; the pH of the proteinase K solution in the step (2) is 7, and the concentration is 100ug / ml.

[0045] (3) Put the glass slide in clean water, touch the specimen on the membrane in the membranous glass slide with a finger with a plastic glove, and rinse with double distilled water to remove large specimens;

[0046](4) Soak the glass slides in the mixed solution B of hydrochloric acid, alcohol and EDTA for...

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Abstract

The invention discloses technology for washing and recycling a membranate glass slide for microdissection. The technology can be implemented according to the following steps: (1) dipping and shaking the membranate glass slide in mixed solution A of boric acid, alcohol and EDTA; (2) washing the membranate by using double distilled water, and transferring the membranate glass slide into prolease K solution to incubate; (3) placing the glass slide into clean water; slightly touching a sample on the membrane by a finger; and washing the membranate glass slide by the double distilled water; (4) dipping the glass slide in mixed solution B of hydrochloric acid, the alcohol and the EDTA; washing the membranate glass slide by using the double distilled water and absolute ethyl alcohol; and finishing the last washing in the absolute ethyl alcohol; and (5) quickly transferring the membranate glass slide onto a super-clean bench; quickly drying the absolute ethyl alcohol; and carrying out ultraviolet radiation at the same time. The technology removes tissues, nucleic acid, albumen and other micromolecular substances remained on the surface of a consumptive material, and can sufficiently maintain the completeness and adhering effect of the membranate glass slide for microdissection.

Description

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Claims

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Application Information

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Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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