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Technique for refining L-tryptophan

A tryptophan and process technology, applied in the field of L-tryptophan refining process, can solve the problems of product content, transmittance and specific rotation failing to meet the quality standard requirements, etc.

Inactive Publication Date: 2009-10-28
河南孟成生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the production of L-tryptophan by fermentation method solves the problem of yield, in the process of fermentation production, partially metabolized water-soluble protein and the remaining protein of raw and auxiliary materials will enter the product, resulting in poor product content and light transmittance. , Specific rotation does not meet the quality standard requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 20g of crude L-tryptophan, which contains 6g of L-tryptophan, is dissolved in a mixed solvent of 100g of water and propanol as a solvent, wherein the solvent contains 83.3g of water and 16.7g of propanol. After the crude product is completely dissolved, react at 50°C for 100 minutes, and filter to obtain a transparent and clear product-containing solution. Pass the solution through a thin-film evaporation device to remove water to obtain wet L-tryptophan. After drying, , obtain 5.1gL-tryptophan fine-tuning (product yield 85%, L-tryptophan mass percentage composition 98.5%), measure its light transmittance and optical rotation by routine method: light transmittance is 90.5% (relatively value), and the optical rotation is -30 degrees, all of which meet the quality standard requirements.

Embodiment 2

[0013] L-tryptophan crude product 25g, crude product contains L-tryptophan 1g, dissolves L-tryptophan crude product with the mixed solvent of 100g water and propanol as solvent, wherein, in the solvent, water 80g, propanol 20g. After the crude product is completely dissolved, react at 65°C for 110 minutes, and filter to obtain a transparent and clear product-containing solution. Pass the solution through a thin-film evaporation device to remove water to obtain wet L-tryptophan. After drying, , obtain 8.8gL-tryptophan fine product (product yield 88%, L-tryptophan mass percentage composition 98.8%), measure its light transmittance and optical rotation by routine method: light transmittance is 91.5% (relatively value), the optical rotation is -31.3 degrees, all of which meet the requirements of quality standards.

Embodiment 3

[0015] The L-tryptophan crude product is 30g, and the crude product contains L-tryptophan 15g, and the mixed solvent of 100g water and propanol is used as a solvent to dissolve the L-tryptophan crude product, wherein, in the solvent, there are 66.7g of water and 33.3g of propanol. After the crude product is completely dissolved, react at 80°C for 120 minutes, and filter to obtain a transparent and clear product-containing solution. Pass the solution through a thin-film evaporation device to remove water to obtain wet L-tryptophan. After drying, , obtain 13.8gL-tryptophan fine product (product yield 92%, L-tryptophan mass percentage composition 99%), measure its light transmittance and optical rotation by routine method: light transmittance is 92% (relatively value), and the optical rotation is -32.5 degrees, all of which meet the quality standard requirements.

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Abstract

The invention pertains to the technical field of biological engineering and particularly discloses a technique for refining L-tryptophan. The mixed solvent of water and propanol is taken as a dissolvent for dissolving L-tryptophan crude product; after the L-tryptophan crude product is dissolved completely, a reaction is conducted on the solution at the temperature of 50-80 DEG C for 100-120min and a transparent and clean solution containing the product is obtained by filtration, wet L-tryptophan is obtained by removing water from the solution, and then fine L-tryptophan product is obtained by drying the wet L-tryptophan. The technique is simple. In the fine L-tryptophan product obtained by the refining technique, the mass percentage of the L-tryptophan is over 98.5 percent, transmittance is higher or equal to 90 percent (a relative value), optical rotation is -30.0 degrees to -32.5 degrees, all meet requirements for quality standard, and product yield is higher than 85 percent.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a process for refining L-tryptophan. Background technique [0002] L-Tryptophan is used as a medical, feed grade or food grade additive, which has high requirements for product content, light transmittance and specific rotation. Although the production of L-tryptophan by fermentation method solves the problem of yield, in the process of fermentation production, some metabolized water-soluble protein and the remaining protein of raw and auxiliary materials will enter the product, resulting in a decrease in product content and light transmittance. , The specific rotation does not meet the quality standard requirements. Therefore, how to develop a refining process for L-tryptophan so that its product content, light transmittance, and specific rotation all meet the quality standard requirements is undoubtedly necessary and even more urgent. Contents of the inven...

Claims

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Application Information

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IPC IPC(8): C07D209/20
Inventor 孟军虎王进全武涛
Owner 河南孟成生物药业股份有限公司
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