Method for keeping activity of probiotic
A technology of probiotics and vitality, which is applied in applications, animal feeds, animal feeds, etc., can solve the problems of probiotics losing their vitality, unable to use heat-sensitive microbial products, and losing efficacy, so as to reduce activity, increase utilization rate, and improve ecology environmental effects
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Embodiment 1
[0013] Embodiment 1 (taking lactobacillus as example)
[0014] Every gram of cornstarch uses 100 micrograms of α-amylase to prepare microporous starch (preparation method with prior art), the reaction time is 12 hours, the reaction temperature is 55 ° C, and the reaction pH is 6.0. Under this condition, better Microporous starch (pore size is about 1 μm. The ratio of the cavity volume of microporous starch to the total volume of starch is greater than 50%), and 100 g of this microporous starch is used to adsorb (also call embedding) 50 g of lactic acid bacteria in its cavity Inside.
[0015] Then use the above-mentioned substances plus the total weight of 100kg of nutrients required by aquatic seedlings (finely crushed particle size less than 0.046μm) as the core material, and use 1kg of chitosan dissolved in 400ml of lactic acid and 40kg of aqueous solution as the wall material to make microcapsules. The most commonly used method for making microcapsules is the spray drying ...
Embodiment 2
[0016] Embodiment 2 (taking lactobacillus as example)
[0017] Every gram of cornstarch uses 100 micrograms of α-amylase to prepare microporous starch (using prior art), the reaction time is 12H, the reaction temperature is 55°C, and the reaction pH is 6.0. Under this condition, better microporous starch can be prepared (the microporous size is about 1 μm. The ratio of the cavity volume of the microporous starch to the total starch volume is greater than 50%). Use this microporous starch 100g to adsorb (also claim to embed) 55g lactobacillus in its cavity.
[0018] Then, the total weight of 100kg of the above-mentioned substances plus various nutrients required by aquatic seedlings (finely crushed particle size less than 0.046μm) is used as the core material, and 1kg of chitosan is dissolved in 400ml of lactic acid and 40kg of aqueous solution is used as the wall material to make microcapsules. The most commonly used method for making microcapsules is the spray drying method. ...
Embodiment 3
[0019] Embodiment 3 (taking lactobacillus as example)
[0020] Every gram of cornstarch uses 100 micrograms of α-amylase to prepare microporous starch (using prior art), the reaction time is 12H, the reaction temperature is 55°C, and the reaction pH is 6.0. Under this condition, better microporous starch can be prepared (The microporous size is about 1 μm. The ratio of the cavity volume of the microporous starch to the total starch volume is greater than 50%). Use this microporous starch 100g to adsorb (also claim to embed) 60g lactobacillus in its cavity.
[0021] Then, the total weight of 100kg of the above-mentioned substances plus various nutrients required by aquatic seedlings (finely crushed particle size less than 0.046μm) is used as the core material, and 1kg of chitosan is dissolved in 400ml of lactic acid and 40kg of aqueous solution is used as the wall material to make microcapsules. The most commonly used method for making microcapsules is the spray drying method. ...
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