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Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit

A technology of hematocrit and sensor chip, applied in the field of determination of hematocrit value of blood sample, concentration of analyte in blood sample, sensor chip and sensor unit, can solve the problem of inability to stably measure Hct value, redox Increased current amplitude fluctuations, etc.

Active Publication Date: 2009-09-09
PHC HLDG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of the sensor chips described in International Publication No. 2005 / 054839 and International Publication No. 2005 / 054840, if the voltage applied between the working electrode and the counter electrode is reduced when the Hct value is measured (Hct value measurement voltage), the amplitude fluctuation of the redox current is aggravated, and the Hct value of the blood sample cannot be stably measured

Method used

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  • Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit
  • Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit
  • Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] A sensor chip A for Hct value measurement was produced. The electrode cores of the working electrode and the counter electrode are made of silver. Using a spacer with a thickness of 100 μm, a blood sample holding portion with a volume of 0.8 microliter (μL) was formed. The effective areas of the active pole and the opposite pole in the blood sample holding part are 1.0mm respectively 2 , 1.8mm 2 , the closest distance between the active pole and the opposite pole is 2.4mm. The surface of the working pole facing the blood sample holding part is the surface of the electrode core body of the working pole. The surface of the counter electrode facing the blood sample holding portion is the surface of a base layer formed of a carboxymethylcellulose (CMC) film disposed on the electrode core of the counter electrode. Regarding the base layer, 0.25% by mass of CMC aqueous solution (manufactured by Daiichi Kogyo Co., Ltd.) was coated on the surface of the electrode core of th...

Embodiment 2

[0148] A sensor chip was produced in the same manner as in Example 1 except that carbon paste (manufactured by Achison Co., Ltd.) was used to form the electrode core of the counter electrode. The above-mentioned three kinds of blood samples are introduced into the blood sample holding parts of different sensor chips, and when the active pole is shown as positive pole and the opposite pole is shown as negative pole, a voltage of 3.0 V or less is applied between the two electrodes, and the voltage at the working pole is measured. and the response current flowing between the opposite poles. The measurement results of the response current are shown in Figures 14-23 of the graph. As shown in each graph, with the sensor chip of Example 2, when expressed in the same manner as above, even if a voltage of 3.0 V or less is applied between both electrodes, a stable and clear signal can be obtained immediately after the voltage application starts. Poor sensitivity detects a response cu...

Embodiment 3

[0150] The same sensor chip as in Example 1 was produced except that a spacer having a thickness of 180 μm was used to form a blood sample holding portion with a volume of 2.5 μL. The above-mentioned three kinds of blood samples are introduced into the blood sample holding parts of different sensor chips, and when the active pole is shown as positive pole and the opposite pole is shown as negative pole, a voltage of 3.0 V or less is applied between the two electrodes, and the voltage at the working pole is measured. and the response current flowing between the opposite poles. The measurement results of the response current are shown in Figures 24 to 28 of the graph. As shown in each graph, with the sensor chip of Example 3, when expressed in the same manner as above, even if a voltage of 3.0 V or less is applied between both electrodes, a stable and clear Poor sensitivity detects a response current reflecting the Hct value of the blood sample.

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Abstract

Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is in contact with the counter electrode but is substantially not in contact with the working electrode, whereby an easily electrolytically oxidizable metal constituting at least a part of the surface of the working electrode is oxidized, and the oxidant in contact with the working electrode is reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the hematocrit value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out, for example, with a sensor chip comprising a working electrode (11), a counter electrode (12), and a blood sample holding part (14) in communication with a blood sample introduction port (16). Theposition of the working electrode (11) in its part (31) is nearer to the blood sample introduction port (16) than the counter electrode in its part (32). The surface of the part (31) contains an easily electrolytically oxidizable metal. A reagent (13) containing an oxidant is disposed in contact with the part (32).

Description

technical field [0001] The present invention relates to a method for measuring the hematocrit (Hct) value of a blood sample, a method for measuring the concentration of an analyte in a blood sample, a sensor chip and a sensor unit suitable for measuring them. Background technique [0002] A sensor chip is used to measure the concentration of an analyte in a blood sample, for example, to measure the glucose concentration (blood sugar level) in blood. [0003] The sensor chip is used to measure the amount of current flowing in the blood sample after the enzyme cycle reaction in which the analyte participates, and calculate the concentration of the analyte according to the amount of current. The amount of current varies based on the Hct value of the blood sample in addition to the analyte concentration. The Hct value of a blood sample fluctuates according to the state of health of the animal providing the blood sample. The human standard Hct value is 39-50% in adult men and 3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/28G01N27/327G01N33/49
CPCG01N27/3274
Inventor 藤原雅树高须贵广池田信中南贵裕
Owner PHC HLDG CORP
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