Breast cancer triple rapid detection kit

A detection kit and breast cancer technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity and specificity, single detection, etc., achieve high sensitivity, reduce interference, and good test repeatability

Active Publication Date: 2017-06-16
珠海博美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the kit can only detect a single indicator, and there are defects of low sensitivity and specificity in the detection process

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, a kind of breast cancer triple rapid detection kit

[0027] Preparation of the triple rapid detection kit for breast cancer

[0028] 1. The preparation method of the antibody microtiter plate is as follows:

[0029] Dilute anti-CA125, HE4 and P-her2 monoclonal antibodies to 40 μg / ml with sodium carbonate buffer solution with a pH of 9.4, add 100 μl / well to a 96-well microtiter plate, and keep the temperature at 4°C Stand overnight, then wash with washing solution, the washing solution is sodium phosphate buffer containing 0.04% dodecyltrimethylammonium chloride, pH is 7.2, spin dry, get coated with anti-CA125, HE4 and 96-well microtiter plate of P-her2 monoclonal antibody.

[0030] 2. The preparation method of the biotin-antibody-coated plate is as follows:

[0031] Mix CA125, HE4 and P-her2 monoclonal antibodies with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin CA125, HE4 and P-her2 monoclonal antibodies; buffer ...

Embodiment 2

[0035] Embodiment 2, a kind of breast cancer triple rapid detection kit

[0036] Preparation of the triple rapid detection kit for breast cancer

[0037] 1. The preparation method of the antibody microtiter plate is as follows:

[0038] Dilute anti-CA125, HE4 and P-her2 monoclonal antibodies to 60 μg / ml with sodium carbonate buffer solution with a pH of 9.4, add 100 μl / well to a 96-well microtiter plate, and keep the temperature at 6°C Stand overnight, then wash with washing solution, the washing solution is sodium phosphate buffer containing 0.04% dodecyltrimethylammonium chloride, pH is 7.2, spin dry, get coated with anti-CA125, HE4 and 96-well microtiter plate of P-her2 monoclonal antibody.

[0039] 2. The preparation method of the biotin-antibody-coated plate is as follows:

[0040] Mix CA125, HE4 and P-her2 monoclonal antibodies with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin CA125, HE4 and P-her2 monoclonal antibodies; buffer ...

Embodiment 3

[0044] Embodiment 3, a kind of breast cancer triple rapid detection kit

[0045] Preparation of the triple rapid detection kit for breast cancer

[0046] 1. The preparation method of the antibody microtiter plate is as follows:

[0047] Dilute anti-CA125, HE4 and P-her2 monoclonal antibodies to 80 μg / ml with sodium carbonate buffer solution with a pH of 9.4, add 100 μl / well to a 96-well microtiter plate, and keep the temperature at 8°C Stand overnight, then wash with washing solution, the washing solution is sodium phosphate buffer containing 0.04% dodecyltrimethylammonium chloride, pH is 7.2, spin dry, get coated with anti-CA125, HE4 and 96-well microtiter plate of P-her2 monoclonal antibody.

[0048] 2. The preparation method of the biotin-antibody-coated plate is as follows:

[0049] Mix CA125, HE4 and P-her2 monoclonal antibodies with activated biotin for labeling, and dialyze to remove unbound biotin to obtain biotin CA125, HE4 and P-her2 monoclonal antibodies; buffer ...

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Abstract

The invention belongs to the biological detection field, and concretely relates to a breast cancer triple rapid detection kit of tumor serum markers CA125, HE4 and P-her2. The breast cancer triple rapid detection kit is mainly composed of an antibody coated well, a biotin antibody coated plate, a serum dilution solution, a horseradish peroxidase, tetramethyl benzidine, hydrochloric acid, a negative reference liquid and a positive reference liquid. The breast cancer triple rapid detection kit can detect the expression level of the CA125, HE4 and P-her2 in postoperative patients or recurrent or metastatic tumor patients, and is of great significance to clinic diagnosis of the breast cancer. The breast cancer triple rapid detection kit reduces interference of other substances in serum, greatly improves the accuracy and the stability of a detection result, and facilitates the early stage diagnosis and the prognosis treatment of the breast cancer patients.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a breast cancer triple rapid detection kit for tumor serum markers CA125, HE4 and P-her2. Background technique [0002] Breast cancer is a genetically heterogeneous disease and the most common malignancy in women. It is reported that there are an estimated 800,000 new cases worldwide each year, and the mortality rate is high. In recent years, breast cancer patients have gradually become younger, which seriously threatens the physical and mental health of women around the world. Currently, mastectomy is the accepted treatment of choice for this disease. Although mastectomy can remove the primary tumor, it cannot detect the possibility of tumor recurrence in the affected area or distant sites due to tumor micrometastasis at the time of diagnosis, and usually requires the administration of cytotoxic agents after surgery as an adjuvant Therapy not only aggravates the suffering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574
CPCG01N33/57415G01N33/577
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner 珠海博美生物科技有限公司
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