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Checking system of special fragment of fluorescence labeling Cannabis sativa L. sexuality gene and method thereof

An inspection system and fluorescent labeling technology, which is applied in the field of gene-specific fragment inspection system, can solve the problems of uncomplicated results, many bands in the electrophoretic spectrum, and many operation steps, and achieve short inspection time, reliable and intuitive results, and simple operation Effect

Inactive Publication Date: 2009-09-09
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Abstract
  • Description
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Problems solved by technology

[0007] From the perspective of detection, AFLP markers, RAPD markers and SCAR markers are mainly analyzed by polyacrylamide gel electrophoresis silver staining technology or agarose electrophoresis EB staining technology in China. The gender-specific fragment detection system has the disadvantages of many steps, time-consuming, low sensitivity, acrylamide, EB dye reagents, etc. are harmful to the health and environment of the experimenters, and require a high level of technical skills for the experimenters.
In particular, there are many bands in the electrophoretic patterns of AFLP markers and RAPD markers, and the results are not easy to judge, which causes certain difficulties for the inspectors when analyzing and identifying, and may cause misjudgments or missed judgments. worst mistake

Method used

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  • Checking system of special fragment of fluorescence labeling Cannabis sativa L. sexuality gene and method thereof
  • Checking system of special fragment of fluorescence labeling Cannabis sativa L. sexuality gene and method thereof
  • Checking system of special fragment of fluorescence labeling Cannabis sativa L. sexuality gene and method thereof

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Embodiment

[0057] The marijuana smoke seized by the anti-drug detachment of Guyuan City, Ningxia Hui Autonomous Region was tested using a fluorescently labeled cannabis sex-specific fragment detection system.

[0058] 1. The DNA in the marijuana sample is extracted by the silica bead method. The reagents and specific operations are as follows:

[0059] 1. Reagent preparation:

[0060] Tris / NaCl (TES) digestion buffer: 100mM Tris-HCL, 500mM NaCl, 2% SDS, 50mM DTT, 0.25mg / mL Proteinase K, 10mM EDTA, PH 8.0

[0061] Adsorption solution: 12g GuSCN dissolved in 10mL 0.1M Tris-HCl (PH6.4), add 0.8mL 0.5MEDTA (PH 8.0) and 0.5mL Triton-X100

[0062] Rinsing solution 1: 12g GuSCN dissolved in 10mL 0.1M Tris-HCl (PH6.4)

[0063] Wash solution 2: 50 mM Tris-HCL, 10 mM EDTA, 0.15 mM NaCl (PH6.5). When used, take 30mL and add 70mL of absolute ethanol.

[0064] Silica bead suspension: Add 0.06 g of silica beads to 1 mL of deionized water.

[0065] 2. DNA extraction steps:

[0066] 1) Take about ...

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Abstract

The present invention provides a checking system of special fragment of fluorescence labeling Cannabis sativa L. sexuality gene, wherein, the checking system is a PCR reacting system which uses the fluorescence labeled DM016 as male primer and uses the fluorescence labeled DM029 as female primer and uses the DNA of Cannabis sativa L. as a formwork. The invention simultaneously provides a checking method of the checking system.

Description

Technical field: [0001] The invention relates to a system and method for detecting gene-specific fragments, in particular to a system and method for detecting specific fragments of cannabis sex genes. Background technique: [0002] Cannabis is an ancient cultivated plant and an important economic crop in many countries in the world. However, it has been listed as a drug by the United Nations Convention Against Narcotics because of its toxic components-tetrahydrocannabinol (THC) and cannabidiol (CBD). . Cannabis is a dioecious plant. The male plant does not contain THC and CBD or contains very little content, while the female plant has relatively high content of both. Therefore, it is believed that the female plant has medicinal value and abuse potential as a drug. However, the male and female plants of marijuana are difficult to distinguish at the seedling stage, and can only be identified after the plants bloom, thus missing the best time to eradicate the original drug pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 裴黎凃政彭建雄季安全张贵芹董林芳徐小玉张颖郭佳冯涛
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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