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Preparation method of haloduric corn germ plasm resource

A corn and germplasm technology, applied in the fields of biotechnology and modern agriculture, can solve problems such as barren land, and achieve the effects of improved salt tolerance, early germination speed, and improved germination rate

Inactive Publication Date: 2009-09-02
天津科润津丰种业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, 15% of the planting soil in the world contains excessive salinity. Even if irrigation technology is used to irrigate water without saline and alkali, the trend of land salinization is still very serious, because the salt contained in the water decreases year after year. Accumulates in the soil, causing tens of millions of hectares of land to go barren every year

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of salt-tolerant maize germplasm resources, comprising the steps of:

[0020] (1). Extract and purify the total DNA of Suaeda salsa, and prepare the seeds of the recipient corn inbred line;

[0021] 1) Preparation of test materials

[0022] The exogenous DNA donor, the halophyte Suaeda salsa, was harvested from the coastal saline land of Tanggu, Tianjin, and the recipient maize inbred line was P2345, a high-yielding and disease-resistant inbred line selected by Tianjin Kerun Jinfeng Seed Industry Co., Ltd.

[0023] 2) Extraction and purification of total DNA of Suaeda salsa

[0024] The improved CTAB method (Wang Yanqin et al., Tarim University Journal, 2007, No. 02) was used to extract the total DNA of the leaves of the halophyte Suaeda salsa, and the extracted crude DNA extract met the requirements of import after purification, and the DNA concentration was 500 μg / ml , measured by ultraviolet spectrum A260 / 230≥2.0, A260 / 280≥1.8;

[0025] (2). T...

Embodiment 2

[0032] A preparation method of salt-tolerant maize germplasm resources, comprising the steps of:

[0033] (1) extract and purify the total DNA of Suaeda salsa, and prepare the seeds of the recipient corn inbred line;

[0034] (2) Cultivate and germinate the seeds of corn inbred lines after sterilizing. After 70% of the seeds germinate and become white, remove abnormal seeds in a sterile environment, place them in a container, and add a concentration of 400 μg / The Suaeda salsa total DNA extract of ml makes the liquid level of the Suaeda salsa total DNA extract 1 centimeter higher than the seeds, and adds the cell penetrating agent acetosyringone so that the final concentration of the acetosyringone is 50 μmol / L, the container was shaken at 80rpm, and cultured at 25°C for 3 days;

[0035] (3) Sowing the germinated seeds of the step (2) in the aseptic sandy soil containing 1% NaCl by mass percentage for screening and cultivating for 7 days, taking the seedlings with strong gro...

Embodiment 3

[0037] A preparation method of salt-tolerant maize germplasm resources, comprising the steps of:

[0038] (1) extract and purify the total DNA of Suaeda salsa, and prepare the seeds of the recipient corn inbred line;

[0039] (2) Cultivate and germinate the seeds of corn inbred lines after sterilizing, and after 90% of the seeds germinate and become white, remove abnormal seeds in a sterile environment, place them in a container, and add a concentration of 600 μg / Suaeda salsa total DNA extract of 1 ml, the liquid level of the total DNA extract of Suaeda salsa is 2 centimeters higher than the seeds, and the cell penetrating agent acetosyringone is added so that the final concentration of the acetosyringone is 150 μmol / L, the container was shaken at 60rpm, and cultured at 30°C for 1 day;

[0040] (3) Sowing the germinated seeds of the step (2) in the aseptic sandy soil containing 1% NaCl by mass percentage for screening and cultivating for 6 days, taking the seedlings with st...

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PUM

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Abstract

The invention discloses a preparation method of a haloduric corn germ plasm resource, which comprises the following steps: (1) the total DNA of purified halophyte suaeda glauca is extracted, and inbred line seeds of corn serving as receptor are prepared; (2), after being sterilized, the inbred line seeds of the corn are cultured and sprouted; and after the inbred line seeds are sprouted, improper inbred line seeds are eliminated, the rest inbred line seeds are put into a container, extracting solution of the total DNA of the halophyte suaeda glauca and a cell osmotic agent are added into the container, the container is oscillated, and rest inbred line seeds are cultivated for 1 to 3 days; and (3), the inbred line seeds cultured to be sprouted are sowed in germfree sandy soil containing NaCl with the mass percent being 1 percent to be filtered and cultured for 6 to 8 days, young seedlings which are strong and have good root systems are moved to a field and are managed according to the normal management for the field, the young seedling are sleeved by bags for autocopuation during the florescence, and grains are harvested. Compared with the unconverted corn inbred line, the haloduric corn germ plasm resource obtained by the method has obviously improved salt endurance. With the germfree sandy soil containing the NaCl with the mass percent being 1 percent, the inbred line seeds sprout one day ahead of time, and the germination percentage of the inbred line seeds in 7 days is enhanced by 15 percent.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and modern agriculture, and relates to a method for preparing germplasm resources of salt-tolerant plants. Background technique [0002] Saline-alkali soil is widely distributed in the world and covers a large area, accounting for about 25% of the total land area. At present, 15% of the planting soil in the world contains excessive salinity. Even if irrigation technology is used to irrigate water without saline and alkali, the trend of land salinization is still very serious, because the salt contained in the water decreases year after year. Accumulated in the soil, causing tens of millions of hectares of land to go barren every year. There are about 2.0×107 hectares of saline-alkali wasteland and 6.7×10 6 Hectares of saline-alkali soil account for about 1 / 4 of the country's land area; in addition, in some irrigated areas, due to unmatched cultivation and irrigation, poor water pipes, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87A01H1/00
Inventor 张烈任丽丽贾天慧
Owner 天津科润津丰种业有限责任公司
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