Method for induction and subculture proliferation regeneration of banana embryogenic callus
A technology for subculture proliferation and embryogenic callus, which is applied in the field of plant tissue culture, can solve the problems of low embryogenic rate and seedling rate, low induction rate of embryogenic callus, long induction time, etc., and achieve embryonic rate High, improved induction efficiency and multiplication efficiency, and low production cost
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[0021] The present invention will be further described below.
[0022] A method for the induction and subculture proliferation and regeneration of banana embryogenic callus, comprising: a culture process for inducing dedifferentiated callus, a culture process for inducing fragile embryogenic callus, and a subsequent culture process for fragile embryogenic callus. Proliferation, induction of embryogenic callus into embryos, induction of mature embryos into seedlings.
[0023] 1) The culture process of induced dedifferentiation callus
[0024] Select the disease-free young sucking buds of bananas that have just been unearthed. After surface treatment, the terminal buds are stripped and cut into thin slices with a thickness of 1-2 mm and a length and width of 0.5-1 cm. They are inoculated on the dedifferentiation callus induction medium for dark culture. The white dedifferentiated callus is obtained, the culture temperature is 25-30 DEG C, and the culture time is 20-30 days. Th...
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