Reagent kit and method for detection of enterocolitis yersinia genus with ring mediated isothermality amplification method

Abstract

The invention discloses a method for detecting food origin enterocolitis Yersinia by using loop-mediated constant temperature PCR technology, belonging to the food sanitation field. The technical proposal is as follows: enterocolitis Yersinia 16S-23S regions are used as target detection sequences, and four specific primers containing the whole region sequences are designed. The invention comprises extraction of four specific primers of enterocolitis Yersinia 16S-23S regions and food origin enterocolitis Yersinia, detection method for loop-mediated constant temperature PCR amplification and interpretation of results. The method fills the gap of a method for detecting food origin enterocolitis Yersinia by using loop-mediated constant temperature PCR amplification technology, and is used for checking enterocolitis Yersinia in import and export foods. The method has the characteristics of low cost, high efficiency, simple operation and convenience.

Description

Technical field [0001] The invention relates to a bacterial inspection technology, in particular to a method for detecting Yersinia enterocolitica, especially food-borne Yersinia enterocolitica, using a loop-mediated constant temperature amplification reaction. Background technique [0002] Yersinia enterocolitica is widely distributed and can be found in raw vegetables, milk and dairy products, meats, soy products, salads, oysters, and shrimps. It also exists in the environment, such as lakes, rivers, soil and vegetation. The bacteria has been isolated from the feces of domestic animals, dogs, cats, goats, squirrels, minks and primates. Around the harbor, many birds including waterfowl and seagulls may be carriers. Pigs have a relatively high rate of infection, with 4% reported in Japan, 3.6% in Canada, and 10-17% in Denmark. In pigs, the bacteria is most easily found in the tonsils. [0003] It is determined that Yersinia as a food-borne disease has a lot of knowledge about its...

AI Technical Summary

Problems solved by technology

[0010] In order to improve the efficiency of food detection, save time, and solve the problem that traditional detection methods cannot meet the growing status of import and export goods, the present invention has finally explored a method for the detection of enterocolitis using loop-mediated constant temperature amplification technology after countless experiments. A method for Yersinia, which has the characteristics of rapid detection, convenience, and low cost

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