Bioreactor and method for animal cell adherent culture

Abstract

The invention provides a zooblast adherent cultured bioreactor and a cell adherent culturing method; a rotary agitating shaft and a textile rope are arranged inside the bioreactor, and the textile rope is used as a cell adherent culturing carrier; a tension fixing device of the textile rope is fixedly connected with the rotary agitating shaft; and the textile rope is arranged on the tension fixing device of the textile rope. The invention takes the textile rope as the culturing carrier and leads zooblast to adhere to and grow on the textile rope. As the rotary agitating shaft drives the textile rope on the tension fixing device to rotate inside the bioreactor, cells adhering to fibers and a culture medium inside the bioreactor are subjected to complete substance exchange, and thorough nutrition that is necessary for cellular growth and metabolism can be supplied. Furthermore, the cells adhering to the fibers can be eluted more conveniently through a digestive agent, and consequently the cells are inoculated to larger bioreactors through a pipeline and continue to be propagation cultivated, thus achieving the goal of large-scale cellular cultivation in linear magnification.

Description

Technical field [0001] The invention relates to an adherent culture technology of animal cells, and in particular to a bioreactor and method for adherent culture of animal cells. Background technique [0002] Such as figure 1 As shown in the US Patent No. 5501971, a fixed-bed basket agitation system is used to fix polyester chips to culture adherent animal cell culture technology: by fixing the polyester chip carrier to the fixed bed basket of the bioreactor In an agitation system, the cells are inoculated into the tank body 102 of the bioreactor for proliferation and culture; for example, the fixed carrier basket 103 is placed at a fixed position in the tank, and the polyester chip carrier 104 is stacked on the fixed position. On the carrier basket 103, the rotation of the stirring shaft 101 promotes the liquid flow. For example, a 5L bioreactor can be loaded with about 150g polyester chips, 5L of culture medium, inoculate a certain amount of cells into the bioreactor, control ...

AI Technical Summary

Problems solved by technology

[0004] 1. Its use in small bioreactors can meet the needs of production, but in larger bioreactors, due to the accumulation of too many polyester chip carriers, the accumulation volume of the carriers becomes larger, and the external medium enters the interior The time of the carrier will be longer, most of the nutrients are consumed by the cells on the outer carrier, and the inner cells get little nutrition, that is, the growth environment of animal cells in the reactor is not uniform, so the utilization of the inner carrier The rate is reduced, resulting in a situation where the yield achieved per unit carrier is reduced

[0005] 2. Unable to achieve linear scale-up of bioreactors

The result is a large amount of labor, easy to infect bacteria, and low production efficiency

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