Harmful algal bloom biological cell surface specific antibody and preparation thereof

Abstract

A harmful red tide organism cell surface specific antibody and a preparation method thereof relate to an antibody. The invention provides a harmful red tide organism cell surface specific antibody and a preparation method thereof. The antibody is an immunoglobulin which is produced by the plasma cells differentiated from B cell and can be combined and reacted with corresponding antigen in a specific way under the incentive of a red tide algae surface antigen substance. Firstly, East China Sea chain prorocentrum Alexandrium whole-cell surface antigen which is not contaminated by the protein in the cell is prepared and then mixed with complete Freund-type adjuvant, later, the mixture is injected into New Zealand white rabbits, and then antigen is injected and injection is enhanced; New Zealand white rabbit ear vein blood is collected 3 to 8 days before the serum antibodies are collected, and later the generation condition of antibodies is detected; the whole rabbit blood is collected 3 to 5 days after the last injection; and after standing and centrifugation, the serum is collected and then packaged and stored. Aiming at the red tide of algae type, corresponding red-tide algae species can be rapidly detected by using the antibody; and the detection limit can reach more than a dozen cells.

Description

technical field [0001] The invention relates to an antibody, in particular to a specific antibody on the cell surface of harmful red tide organisms with high specificity and sensitivity and a preparation method thereof. Background technique [0002] At present, the preparation of antibodies against harmful red tide algae has been reported. [0003] Qi Haigang et al. (Qi Haigang, Mi Tiezhu, Xin Zeyu, et al. Preparation of H. akashiwa antibody and establishment of enzyme-linked immunoassay [J]. Oceanographic Journal, 2006, 28(5): 167-172) reported the preparation of red tide Heterocurvium Antibodies. [0004] Xiang Junjian et al. (Xiang Junjian, Ling Qinjie, Lu Songhui, et al. Preparation and specificity analysis of four polyclonal antibodies against red tide algae [J]. Journal of Jinan University, 2005, 26(5): 700-704) prepared four Red tide algae antibody serum and red tide algae monoclonal antibody. [0005] Xiang Junjian et al. (Xiang Junjian, Zhu Xiaobing, Lu Songhui, ...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a harmful red tide biological cell surface specific antibody and its preparation to solve the problems that the existing harmful red tide biological cell surface antibody is easily polluted by intracellular protein and it is difficult to obtain highly specific antibodies. method

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