Apparatus for designing nucleic acid amplification primers, program for designing primers and server apparatus for designing primers

A primer design and server technology, applied in instrumentation, computing, sequence analysis, etc., can solve problems such as inability to fully obtain target amplification products

Inactive Publication Date: 2009-07-15
SHIMADZU SEISAKUSHO CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the target amplification product cannot be sufficiently obtained

Method used

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  • Apparatus for designing nucleic acid amplification primers, program for designing primers and server apparatus for designing primers
  • Apparatus for designing nucleic acid amplification primers, program for designing primers and server apparatus for designing primers
  • Apparatus for designing nucleic acid amplification primers, program for designing primers and server apparatus for designing primers

Examples

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Embodiment

[0276] Examples are shown below to specifically explain the present invention, but the present invention is not limited to the following examples. Among them, the candidate primers will be abbreviated as primers below.

[0277] In the following examples, the primer design program of the present invention is used to design primers for multiplex PCR. The primers judged to be the best by the program of the present invention and the primers with a lower priority are used to actually perform multiplex PCR. In addition, under the condition that the primers are wrong but not bound to each other, the effect of the combination of the PCR product and other primers on the efficiency of PCR is tested. The test is performed by a multiplex PCR system that amplifies two single nucleotide polymorphism (SNP) sites. The general content from primer design to inspection is shown below.

[0278] For the second SNP site, candidate primers are independently selected by the primer design software for si...

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Abstract

It is intended to provide an apparatus for designing primers, whereby an appropriate primer never forming any undesired nucleic acid fragment due caused by the binding of the primer to an unintended part in a nucleic acid amplification reaction can be designed, a program for designing primers and a server apparatus for designing primers. Namely, a program for designing primers aiming at computerizing the procedure for determining a series of primers to be used in amplifying a plural number of sequences in a nucleic acid, wherein a series of primers to be used in amplifying a plural number of sequences in a nucleic acid are determined by, for each potential combination in selecting two sites from among multiple sites to be amplified, computing and scoring the complementary between the sequence of a candidate primer for one of the two sites and the sequence of an amplification product obtained at the other sites of the two sites.

Description

Technical field [0001] The present invention relates to a primer design device for nucleic acid amplification, a primer design program, and a primer design server device (Device for Designing primer, Program for Designing primer, and Server for Designing primer). The present invention relates to a computing device, a program, and a server device for designing primers used in a reaction to amplify a necessary base sequence from an analysis target nucleic acid. Background technique [0002] As a reaction for simultaneously amplifying two or more nucleic acids, multiplex PCR (Polymerase Chain Reaction) is known. As a technique for typing single nucleotide polymorphisms (SNPs) using multiplex PCR, there is a patent document: Japanese Patent Application Laid-Open No. 2002-300894 "Single Nucleotide Polymorphism Typing Method" and the like. As shown in this technique, multiplex PCR is a useful technique for efficiently amplifying a small amount of DNA extracted from a small amount of bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/00C12N15/09G16B20/20
CPCG06F19/22G16B30/00G16B20/20
Inventor 中村祐辅此下龙绪方是嗣
Owner SHIMADZU SEISAKUSHO CO LTD
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