Rooting method for tissue culture of Chinese fir

Abstract

The invention provides a fir tissue culture rooting method. Small fir multiplication subculture seedlings are cut and transplanted to a fir rooting culture medium for culturing and rooting tissue, and the fir rooting culture medium comprises an improved MS culture medium, 0.4-0.8mg / L of ABT1#, 0.1-0.5mg / L of IBA and 2-6mL / L of root sun diluent. After the fir rooting culture medium is adopted, the rooting time of firs is shortened to 16 to 22 days; after 22 days of culture, the highest rooting rate achieves 96 percent, and nursery stocks grow rapidly so as to decrease the culture cost of the seedlings and realize the industrialized production of the firs.

Description

technical field [0001] The invention relates to a Chinese fir tissue culture rooting method, in particular to a method for tissue culture rooting using the Chinese fir rooting medium composed of an improved MS medium and hormones. Background technique [0002] Chinese fir (Cunninghamia lanceolata (lamb.) Hook) is a species of economic forest with wide adaptability and excellent material quality. Since Chinese fir is a cross-pollination tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the Chinese fir parent. However, conventional asexual reproduction methods such as cuttings, layering or grafting have slow breeding speeds and are difficult to meet the growing market demand. For this reason, Chinese fir will obtain a large amount of excellent clone seedlings in a short period of time, only by plant tissue culture technology to solve the problem. The tissue culture method is a rapid vegetative p...

AI Technical Summary

Problems solved by technology

In the process of commercial industrialization of Chinese fir tissue culture, problems such as slow rooting, low rooting rate, and slow growth rate have always hindered the process of industrialized seedling production of Chinese fir

Therefore, the applicant intends to provide a novel Chinese fir rooting medium to solve the problems of difficult rooting and low rooting rate of Chinese fir in the existing Chinese fir culture technology

Images