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Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method

A technology of Ganoderma lucidum polysaccharide and phenol is applied in the field of determination of Ganoderma lucidum polysaccharides, which can solve the problems such as inability to accurately react active active ingredients of Ganoderma lucidum polysaccharides, inappropriate determination of Ganoderma lucidum products, uneven product quality, etc. Actionable effect

Inactive Publication Date: 2009-07-08
宋秋兰
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] With the in-depth research on the extraction methods of Ganoderma lucidum polysaccharides, the types and quantities of Ganoderma lucidum polysaccharide products on the market are also increasing, and the quality of their products varies, leading to market confusion
In particular, the country does not have corresponding testing standards for the quality of Ganoderma lucidum polysaccharide products, and it is determined by referring to "GB / T15672-1995 Determination of Total Sugar Content in Edible Fungi". The content of the active and active ingredient Ganoderma lucidum polysaccharide
> (Edited by Wang Guangya, Beijing: China Light Industry Press, 2002.1) has disclosed several methods for measuring the efficacy components of health products, among which "spectrophotometric determination of Lycium barbarum polysaccharide content" is of great importance for the determination of Ganoderma lucidum spores, The content of Ganoderma lucidum polysaccharide in the fruit body and mycelium is of reference significance, however, it is not suitable for the determination of the content of Ganoderma lucidum polysaccharide in Ganoderma lucidum products

Method used

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  • Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method
  • Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method
  • Method for measuring ganoderma polyoses content in ganoderma products by sulfuric acid-phynol method

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Experimental program
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Embodiment 1

[0029] 1. Remove interfering components: Weigh 5.0083g, 5.0061g, 5.0044g, 5.0054g of Zizhi fungus powder, dissolve in 100ml of 80% ethanol, heat and reflux in a water bath at 60°C for 30min, centrifuge at 4500r / min for 10min, discard the supernatant, Collect the residue. Dissolve in 100ml of 80% ethanol, repeat the above steps twice, to remove interfering components such as monosaccharides, disaccharides and oligosaccharides.

[0030] 2. Extract polysaccharide: Dissolve the above residue with 200ml of distilled water, heat and reflux in a water bath at 95°C for 2 hours, and set the volume to 250ml after cooling (V 1 milliliters), to obtain the extract, mix well, filter, and collect the filtrate to be the sample solution, measure 10ml (V 2 milliliter) sample solution, dilute 10 times, and set the volume to 100ml (V 3 mL), which is the solution to be tested.

[0031] 3. Determination of Ganoderma lucidum polysaccharide content by sulfuric acid-phenol method:

[0032] 3.1. Dr...

Embodiment 2

[0056] 1. Remove interfering components: weigh 5.0061g, 5.0057g, 5.0044g, 5.0035g of Zizhi fungus powder, dissolve in 100ml of 85% ethanol, heat and reflux in a water bath at 61°C for 30min, centrifuge at 3000r / min for 10min, discard the supernatant, Collect the residue. The residue was dissolved in 100ml of 85% ethanol, and the above steps were repeated twice to remove interfering components such as monosaccharides, disaccharides and oligosaccharides.

[0057] 2. Extract polysaccharide: Dissolve the above residue with 200ml of distilled water, heat and reflux in a water bath at 96°C for 1.5h, and set the volume to 250ml after cooling (V 1 milliliters), to obtain the extract, mix well, filter, and collect the filtrate to be the sample solution, measure 10ml (V 2 milliliter) sample solution, dilute 10 times, and set the volume to 100ml (V 3 mL), which is the solution to be tested.

[0058] 3. Determination of Ganoderma lucidum polysaccharide content by sulfuric acid-phenol m...

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Abstract

The invention relates to a method for determining ganoderma lucidum polysaccharide, in particular to the method for determining the content of the ganoderma lucidum polysaccharide in a glossy ganoderma product by a sulfuric acid-phenol method. The method for determining the ganoderma lucidum polysaccharide in the glossy ganoderma product by the sulfuric acid-phenol method comprises the following steps: firstly, using 80 to 85 percent of alcohol to precipitate to remove small molecular impurities such as monosaccharide, disaccharide, oligosaccharide, and so on; secondary, using distilled water to dissolve and extract polysaccharide; and finally, using the sulfuric acid-phenol method to determine the content of the ganoderma lucidum polysaccharide in the glossy ganoderma product. The method has the advantages of strong operability, accurate determination result, high sensitivity, good reproducibility, carrying out determination in general routine laboratories, and so on.

Description

technical field [0001] The invention relates to a determination method of ganoderma lucidum polysaccharides, in particular to a sulfuric acid-phenol method for determining the content of ganoderma lucidum polysaccharides in ganoderma products. Background technique [0002] Ganoderma lucidum (Leyss ex Fr.) Krast.) belongs to Basidiomycetes, Polyporaceae, Ganoderma higher fungi. Traditional Chinese medicine believes that Ganoderma lucidum is sweet in taste, warm in nature, non-toxic, and can replenish the five swollen qi of the heart, liver, lung, spleen, and kidney. Modern medical research also shows that the main active ingredient of Ganoderma lucidum is Ganoderma lucidum polysaccharide, which has a wide range of pharmacological activities, detoxification, anti-radiation, improving the ability of the liver and bone marrow to synthesize DNA and RNA, anti-tumor, and enhancing body immunity. [0003] Ganoderma lucidum polysaccharides are widely present in Ganoderma lucidum spo...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/34
Inventor 宋秋兰
Owner 宋秋兰
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