Isolation in vitro and amplification method for adult rat heart microvascular endothelial cell
A technology of endothelial cells and microvessels, applied in artificial cell constructs, animal cells, vertebrate cells, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment approach
[0023] 1. Isolation and culture of endothelial cells
[0024] Get 2 (150-200g) SD rats, 3% pentobarbital sodium (30mg / Kg) intraperitoneal anesthesia, open the chest cavity, remove the heart, wash the heart cavity with PBS buffer until the flushing fluid is clear. After removing the atrium and right ventricle, cut the left ventricle along the front wall, take it out quickly after 75% alcohol inactivation for 30 seconds, and place it in PBS buffer to remove residual alcohol. Cut off the inner and outer membranes, shred the myocardial tissue, digest with 0.2% type II collagenase for 5 minutes, add 0.1% trypsin to continue the digestion for 5 minutes, and stop the digestion with DMEM medium containing 20% newborn bovine serum. Collect the digestive juice and centrifuge at 1000r / min for 10min. Discard the supernatant, suspend the cells with DMEM culture medium (containing 20% newborn bovine serum, penicillin 100 IU / ml, streptomycin 100 μg / ml), inoculate in a petri dish covered...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com