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P16<INK4a> double antibody sandwich ELISA kit

A detection kit and immunoadsorption technology, which is applied in the field of immunological reagent products, can solve the problems of low specificity and low accuracy, and achieve the effects of low detection cost, easy use, and rapid detection results

Inactive Publication Date: 2009-06-03
邹先进
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a P16 assay with high sensitivity, specificity and accuracy, aiming at the insufficient sensitivity, specificity and accuracy of the existing enzyme-linked immunosorbent assay kits for cancer screening. INK4a Double Antibody Sandwich ELISA Kit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] 1. Materials and methods:

[0007] 1. Specimen collection: 1356 cases of cervical specimens were collected. The women who received the experiment were between 24 and 70 years old, all married and had no history of cervical surgery. In all cases, the exfoliated cells were extracted by swabbing the cervix for 2 weeks with a sterile cotton swab, and then the cervical exfoliated cells were obtained with a sterile cell brush for TCT examination. The cervical exfoliated cells extracted from cotton swabs and the cervical exfoliated cells fixed in TCT preservation solution were all used in P16 INK4a ELISA kit for detection of p16 INK4a protein expression. Under colposcopy, cervical biopsies were collected for histopathological examination. Sputum samples were collected from 20 lung cancer patients and 15 normal subjects.

[0008] 2. P16 in exfoliated cells INK4a Protein extraction: Take 5ml of cell preservation solution, centrifuge and wash twice with normal saline, add 3...

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PUM

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Abstract

P16<INK4a> double antibody sandwich ELISA kit comprises avidin, peroxidase compound, coloration liquid, reaction stop solution, positive reference substance, antibody diluent and concentrated cleaning solution and is characterized in that the kit also comprises ELIAS plate coated by P16<INK4a> polyclonal antibody and P16<INK4a> monoclonal antibody marked by biotin. The invention has the advantages that the double antibody sandwich ELISA kit is used for screening cervical carcinoma, wherein, the sensibility is 87.80%, the specificity is 92.43%, and the general coincidence rate is 91.59% and the negative predictive value is 97.16%. The kit is simple to be used, the detection result is obtained fast and is correct and objective; a plurality of samples can be detected simultaneously; in addition, the kit is applicable for the cancer screening for a large sample population, and the detection cost is lower than the cost of the traditional caner screening method.

Description

technical field [0001] The invention relates to the field of immune reagent products. Background technique [0002] P16 INK4a The protein is a tumor suppressor gene product, but it is overexpressed in various human tumors, especially almost 100% in cervical cancer and precancerous lesions, but not expressed in normal cervical epithelial cells. At the same time, P16 INK4a It is also expressed in various tumors such as lung cancer and esophageal cancer. P16 INK4a Is a variety of human tumor-associated markers. In recent years, immunohistochemical methods have been used to detect P16 on pathological tissue sections at home and abroad. INK4a , use it for scientific research or for auxiliary pathological diagnosis. At present, there are enzyme-linked immunosorbent assay kits at home and abroad, which include avidin-peroxidase complex, chromogenic solution, reaction termination solution, positive control substance, antibody diluent, concentrated washing solution, but it is u...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 邹先进丁莉
Owner 邹先进
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