Chinese medicine DNA detection kit and identification method

Abstract

The invention relates to the Chinese medicine detection technology field and belongs to a Chinese medicine DNA detection kit, comprising an identification reaction system, a positive control reaction system and a negative control reaction system; both the identification reaction system and the positive control reaction system contain buffer solution, 12.5mM dNTP, one type of 0.1mM deer penis primer, fritillary primer and root of fangji primer, one type of Taq DNA polymerase, deer penis, fritillary and root of fangji DNA, sample DNA to be detected, and double-distilled water; the negative control reaction system contains buffer solution, 12.5mM dNTP, one type of 0.1mM deer penis primer, fritillary primer and root of fangji primer, one type of Taq DNA polymerase, bullwhack, false fritillary and fake root of fangji DNA, and double-distilled water. The Chinese medicine DNA identification method comprises steps such as designing deer penis, fritillary, root of fangji mitochondrial DNA three-pair specific oligonucleotide primer, artificial synthesis, fixing reaction procedures and result judgment, and the like; the Chinese medicine DNA identification kit can accurately identify the specificity of Chinese medicinal materials; and the identification method has the advantages of simplicity, rapidness, and reliable detection result, etc.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, more specifically, a traditional Chinese medicine DNA detection kit and identification method. Background technique [0002] The supervision and inspection of easily mixed varieties of Chinese medicinal materials is one of the key tasks of the drug inspection department every year. At present, chemical, infrared, ultraviolet, liquid chromatography and other analytical methods are widely used in the detection of Chinese medicinal materials at home and abroad. The above methods are different for the same family and genus. The specificity of the identification of easily mixed medicinal materials is poor, and infrared, ultraviolet, liquid chromatography and other methods have the disadvantages of low specificity for substances with similar chemical structures, cumbersome operations, and high costs, which are difficult to meet the identification of easily mixed varieties ...

AI Technical Summary

Problems solved by technology

[0002] The supervision and inspection of easily mixed varieties of Chinese medicinal materials is one of the key tasks of the drug inspection department every year. At present, chemical, infrared, ultraviolet, liquid chromatography and other analytical methods are widely used in the detection of Chinese medicinal materials at home and abroad. The above methods are different for the same family and genus. The specificity of the identification of easily mixed medicinal materials is poor, and infrared, ultraviolet, liquid chromatography and other methods have the disadvantages of low specificity for substances with similar chemical structures, cumbersome operations, and high costs, which are difficult to meet the identification of easily mixed varieties of traditional Chinese medicine.

Due to the limitations of the existing analysis methods, it is difficult to make an accurate judgment on the authenticity and falsehood of the existing easily mixed varieties of traditional Chinese medicine.

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