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A technology of dicarboxylic acid and tricarboxylic acid, which can be used in biocides, animal repellents, plant growth regulators, etc., and can solve problems such as insufficient activity
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[0018] A beverage containing fruit components was mixed with different concentrates of citric acid and subsequently inoculated with conidia of the fungus Paecilomyces mutans. Subsequently, these inoculated beverage samples were treated with different DMDC concentrates and incubated at 27°C for two weeks. In subsequent evaluations, it became clear that 250 ppm of DMDC alone and 30,000 ppm of citric acid alone were insufficient to prevent the growth of Paecilomyces villiformis, while in combination, as little as 200 ppm of DMDC and 3000 ppm of citric acid was sufficient to prevent fungal growth (see Table 1).
[0019] Table 1
[0020] MHC [ppm] DMDC (pure active compound) >250 Citric acid (pure acidulant) >30000 DMDC / citric acid combination 200 / 3000
[0021] The synergy was determined according to the method of Kull et al. (F.C. Kull, P.C. Eismann, H.D. Sylvestrowicz, R.L. Mayer, Applied Microbiology 9, 538 to 541, 1961). In this approach, ...
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