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Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof

A molecularly imprinted membrane and sensing electrode technology, applied in the direction of material impedance, etc., can solve the problems of high preparation cost of molecularly imprinted membrane electrode, difficult to realize a large number of sample detection, complicated electrode processing process, etc., to achieve favorable detection effect and avoid oxidation. Interference of the reduction reaction, avoiding the effect of thermal polymerization

Inactive Publication Date: 2012-08-22
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the working electrode is a glassy carbon electrode, the cost is high, the operation is cumbersome when preparing the molecularly imprinted membrane on the surface of the working electrode, the electrode treatment process is complicated, and multiple elutions are required during the preparation of the molecularly imprinted membrane. The process takes a long time
In short, the preparation cost of the molecularly imprinted membrane electrode is high, the preparation time is long, and it is difficult to detect a large number of samples, so it has great limitations in practical applications.

Method used

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  • Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof
  • Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof
  • Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof

Examples

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Effect test

preparation example Construction

[0040] The preparation method of the conductivity-type sensing electrode molecularly imprinted membrane for detecting chloramphenicol involved in the following examples comprises the following steps:

[0041] Step 1, preparation of chloramphenicol molecularly imprinted membrane: Weigh chloramphenicol template molecules and functional monomers and dissolve them in a solvent, mix them uniformly by ultrasonic vibration, add a cross-linking agent, and then add an adhesive after mixing uniformly by ultrasonic vibration, and then Mix uniformly by ultrasonic vibration, then add the initiator, mix uniformly by ultrasonic vibration, pass nitrogen gas to remove oxygen, add the solution prepared by the above operation dropwise to the reaction area of ​​the working electrode of a screen printing electrode in the conductivity type sensing electrode, cover The glass slide is covered, and then the screen-printed electrode is placed flat in a petri dish, and a mixture of ice and dry ice is pla...

Embodiment 1

[0055] The conductive silver paste is printed on the electrode substrate with the conductive silver paste and the substrate of the terminal, the surface of the electrode connection is coated with a layer of PVC insulator, and the working electrode at the lower end is coated with a layer of conductive graphite by sputtering graphite method. Soak in 50% nitric acid, and then rinse with distilled water for 3 times. Let dry and store at 4°C for later use.

[0056] Weigh 0.05g of chloramphenicol and 70μl of methacrylic acid functional monomer and dissolve in 380μl of tetrahydrofuran solvent, mix ultrasonically for 10 minutes, add ethylene glycol dimethacrylic acid crosslinking agent, mix ultrasonically for 10 minutes, and then add to the reaction system 70 μl of water-based polyurethane adhesive, after ultrasonic mixing for 10 minutes, add 0.02 g of initiator azoisobutylcyanide, after ultrasonic mixing for 20 minutes, nitrogen gas for 5 minutes, then add 10 μl of the prepared mixtu...

Embodiment 2

[0064] In the preparation process of the chloramphenicol molecularly imprinted membrane, the ultraviolet polymerization time was continuous irradiation for 30 minutes, and other conditions remained unchanged. During the detection process, the preparation of the standard curve was the same as in Example 1, and chicken meat was used as the detection sample.

[0065] Sample processing: Take 5 chicken samples confirmed by high performance liquid chromatography-mass spectrometry to not contain chloramphenicol residues, take 1 gram of meat samples, put them into the sample cup of the tissue homogenizer, add 10 ml of 0.1M pH to 7.2 Phosphate buffer solution, crushed and homogenized, transferred to a 15ml centrifuge tube, added 1ml of chloramphenicol standard solution containing 40μg / L, then extracted with 6ml of ethyl acetate, shaken and mixed for 15min, Collect the organic phase containing chloramphenicol, evaporate and remove the organic solvent at 40°C and 240mbar, and finally add...

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Abstract

The invention relates to a conductive type sensing electrode for detecting chloramphenicol in the field of biosensors and a method for preparing a molecular engram membrane of the chloramphenicol. In the invention, the conductive type sensing electrode is formed by arranging two screen painting electrodes in parallel. The method for preparing the molecular engram membrane of the chloramphenicol comprises the following steps: template molecules are embedded in a direct in-situ polymerization process of polymer fluid on the surfaces of the disposable screen painting electrodes, and then the template molecules are eluted so that cavities with set sizes and shapes and functional groups in confirmed arrangement are formed in the molecular engram membrane and have memorability to stereoscopic structures of the template molecules. Chloramphenicol molecules in a sample to be detected can be combined with the specificity of the cavities so as to change the electrochemical properties of the molecular engram membrane, and increase the conductivity and the membrane conductance of the molecular engram membrane. The processing method of the invention has simplicity, convenience and low cost, and the conductive type sensing electrode made of the screen painting electrodes has convenient operation, high response time and long service life when used for detecting the chloramphenicol.

Description

technical field [0001] The invention relates to an electrode in the technical field of detection and a preparation method thereof, in particular to a conductivity-type sensing electrode for detecting chloramphenicol and a preparation method of a molecular imprinted membrane thereof. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is a kind of efficient broad-spectrum antibiotic, is widely used in the treatment of various infectious diseases of animals, but, chloramphenicol has serious side effect, can cause human's aplastic anemia and granular leukocyte Deficiency and other diseases. In addition, long-term micro-intake of chloramphenicol not only makes Escherichia coli, Salmonella, etc. produce drug resistance, but also causes the normal flora of the body to become imbalanced, making people susceptible to various diseases. If chloramphenicol is used in food animals Therefore, the residue of chloramphenicol in animal food poses a potential hazard to human h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/02
Inventor 柴春彦李锋王莉刘国艳李为强
Owner SHANGHAI JIAOTONG UNIV
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