Cinobufacini extract and preparation method thereof
A technology of extract and cinobufacin, which is applied in the directions of drug combination, pharmaceutical formula, and medical preparations containing active ingredients, etc., can solve the problems of reduced curative effect, toxic and side effects, and low content of active ingredients of ineffective substances.
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Embodiment 1
[0062] Take 1kg of dried toad skin, add 10L water to decoct for 45 minutes, that is, the first decoction; after the first decoction, add 5L water to decoct the dregs, that is, the second decoction; combine the two decoction liquids, filter, and cool the filtrate to 30°C , adsorbed on D101 macroporous adsorption resin column, first eluted with 6L of water, then 5L of 70% ethanol, collected the ethanol eluate, recovered the ethanol in the eluate, concentrated, dried, and obtained cinobufacin extract 16.5g, the total amount of sinobufonigen in the extract is 31.2mg, and the total amount of redibufagenin is 20.8mg.
Embodiment 2
[0064] Take 1kg of dried toad skin, add 7L water to decoct for 45 minutes, that is, the first decoction; ℃, adsorb on AB-8 macroporous adsorption resin column, first elute with 7L of water, then elute with 6L of 60% ethanol, collect the ethanol eluate, recover the ethanol in the eluate, concentrate, dry, and get Hua 15.3g of bufobufoni extract, the total amount of sinobufonitoxin in the extract is 22.9mg, and the total amount of redibufagenin is 15.6mg.
Embodiment 3
[0066] Take 1kg of dried toad skin, add 10L of water, and decoct for 30 minutes, that is, the first decoction; ℃, adsorbed on NKA-9 macroporous adsorption resin column, first eluted with 5L of water, then 4L of 80% ethanol, collected the ethanol eluate, recovered the ethanol in the eluate, concentrated, dried, and obtained C. toads The extract is 18.4g, and the total amount of sinobufagin in the extract is 36.5mg, and the total amount of redibufagenin is 24.2mg.
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