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Use of high-purity forsythin in preparing bacteriostasis, antivirus medicine

A technology of forsythiaside and forsythiaside monomer, which is applied in the direction of antiviral agents, antibacterial drugs, and drug combinations, and can solve the research on antibacterial, antiviral, antipyretic and anti-inflammatory pharmacological activities of forsythiaside and other problems, to achieve good antibacterial and antiviral effects, inhibit proliferation, and improve immunity

Active Publication Date: 2011-09-28
SHANDONG NEWTIME PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no existing technology to study the antibacterial, antiviral, antipyretic and anti-inflammatory pharmacological activities of this high-purity forsythiaside

Method used

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  • Use of high-purity forsythin in preparing bacteriostasis, antivirus medicine
  • Use of high-purity forsythin in preparing bacteriostasis, antivirus medicine
  • Use of high-purity forsythin in preparing bacteriostasis, antivirus medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 (antipyretic, anti-inflammatory)

[0014] 1.1 Purpose of the experiment

[0015] Observe the use of high-purity forsythiaside in antipyretic and anti-inflammatory aspects.

[0016] 1.2 Experimental materials

[0017] 1.2.1 Drugs

[0018] Test drug: high-purity forsythiaside: the active ingredient extract of forsythia; purity > 90%; provided by Shanghai Yusen New Drug Development Co., Ltd. Physical and chemical properties: This product is yellow powder. Storage conditions: 4°C, protected from light. Batch number: 060425.

[0019] Three dose groups (hereinafter referred to as high-purity groups) were set up in the test. ① Rat administration preparation method: according to 10ml / (kg d) tail vein injection administration, the low, middle and high dose groups are respectively equivalent to 3, 6, 12 times of clinical daily dosage; ② Mice administration preparation method : Administered at 10ml / (kg·d), the low, middle and high dose groups are respectively e...

Embodiment 2

[0063] Embodiment 2 (enhancing immunity test)

[0064] 2.1 Purpose of the experiment

[0065] Observe the use of high-purity forsythiaside in enhancing immunity.

[0066] 2.2 Experimental materials

[0067] With 1.2 experimental material in embodiment 1

[0068] 2.3 Data processing

[0069] Same as 1.3.

[0070] 2.4 Methods and Results

[0071] 2.4.1 Effect of high-purity forsythiaside on the carbon clearance ability of mice

[0072] The grouping was the same as in 1.4.1. Continuous tail vein injection of 10ml / kg was administered for 1 week. The mice in each group were injected with 0.1ml of 20% India ink through the tail vein respectively, and 20ul of blood was collected at 1 minute and 5 minutes after the injection and placed in 2ml0. Mix in 1% Na2CO3 solution, measure the OD value at 680nm, and calculate the K value according to the following formula.

[0073] Formula: K=(logOD1-logOD5) / (t5-t1)

[0074] The results showed that there was a significant difference betw...

Embodiment 3

[0087] Embodiment 3 (in vitro antiviral test)

[0088] 3.1 Virus multiplication:

[0089] Inoculate influenza type A on MDCK cells, add maintenance solution and culture at 37°C, 5% CO2, more than 90% of the lesions appear after 96 hours, freeze and thaw 3 times, blow and centrifuge, quantitatively aliquot, and store in -80°C refrigerator for later use .

[0090] 3.2 Determination of virus virulence:

[0091] Make a 10-fold serial dilution of the spare influenza A virus with the maintenance solution, repeat 3 wells vertically, inoculate horizontally on the monolayer cells in a 96-well plate, culture at 37°C, 5% CO2, observe the lesions every day, 48 After -96h, suck and discard the liquid in the plate wells, add 100ul of 1% neutral red, place at 37°C for 2h, discard the dye solution, fully elute excess dye with washing solution, add 100ul of decolorizing solution, decolorize at room temperature for 10min, and use enzyme The standard instrument measures the OD value at a wave...

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Abstract

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purityforsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to the use of high-purity forsythiaside active ingredients in the preparation of antipyretic, anti-inflammatory, immunity-improving, bacteriostatic and antiviral drugs. Background technique [0002] Forsythoside A is an active ingredient extracted from the plant Forsythia (Fructus Forsythiae) in the family Oleaceae. In the past, forsythin was used as an index to investigate the extraction process of forsythia. In recent years, with the deepening of research on the active ingredients of forsythia, it was found that forsythin has no antibacterial activity, and forsythiaside exerts antibacterial activity. Almost all of forsythiasides are affected by forsythiaside A, but it is difficult for forsythiaside A to exist stably alone, so it is customary to use forsythiaside A The active ingredient is called forsythiaside. The structural formula of forsythiaside (A) is as f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7032A61K36/634A61P31/04A61P31/12A61P31/16A61P29/00A61P37/04
Inventor 玄振玉王勇
Owner SHANDONG NEWTIME PHARMA
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