Method and apparatus for monitoring macromolecule conformation transformation and biochemical reaction of target and probe

Abstract

The invention discloses a method and a device for observing the conformation transition of macromolecules and the biochemical reaction between target molecules and probe molecules. The method comprises the steps as follows: the macromolecules and one or more of the probe molecules which are different or identical are arranged on single side surfaces of various micro-cantilevers in a micro-cantilever array; the incident parallel rays emitted from a light source reach the micro-cantilevers, and the reflection rays are received by an optical receiver assembly to form an array of reflection ray spots corresponding to the micro-cantilevers one by one; when the conformation transition of macromolecules occurs or the combination of the probe molecules and the target molecules present in a solution to be tested occurs, the micro-cantilevers bend and deform with the deflection of the reflection rays, and accordingly, the positions of the corresponding reflection ray spots change; and according to the position change and the displacement of the reflection ray spots corresponding to each micro-cantilever, the conformation transition of the macromolecules can be learned, whether the target molecules corresponding to the probe molecules are present or absent in the solution to be tested can be determined, and the concentration of the target molecules present in the solution can be obtained. The invention can realize the simultaneous observation of the displacement signals of a plurality of micro-cantilevers, and at the same time eliminate the noise signals due to the variation of the refraction index and the change of the intensity of a light source. The invention has the advantages that the effects of vagabond rays can be greatly reduced by controlling the measurement of the light beam polarization state, and the measurement precision is improved.

Description

technical field [0001] The invention relates to a method and a device for monitoring macromolecular conformation transitions and biochemical reactions, in particular to a method and a device for monitoring macromolecular conformation transitions and biochemical reactions between target molecules and probe molecules by using a micro-cantilever beam array. Background technique [0002] Microcantilevers modified with macromolecules or probe molecules after surface chemical treatment, when environmental changes (such as temperature, pH, etc.) lead to conformational changes of macromolecules or when biochemical reactions occur on the surface (such as When the target molecule reacts biochemically with the probe molecule on the micro-cantilever), the surface stress (that is, the surface energy) of the micro-cantilever will change, resulting in bending deformation of the micro-cantilever. By detecting this deformation, we can know the whole Information about the progress of molecula...

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Problems solved by technology

However, under the light path of this invention, there are the following disadvantages: when the buffer solution in the reaction vessel is replaced by the environmental solution or the solution to be tested that causes the molecular conformation to change, the reflected beam will be deflected due to the slight change in the refractive index of the solution, that is, the detection The signal is coupled with the noise signal caused by the change of the refractive index of the solution (Bumbu G G, Wolkenhauer M, et al. Micromechanical cantilever technique: A tool for investigating the welling of polymer brushes [J]. Langmuir 2007, 23: 2203-2207); and only Applicable to a single micro-cantilever, that is, one experiment can only detect the deformation signal of one micro-cantilever

However, this detection method is greatly affected by stray light and light intensity changes of the light source.

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