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Aculeus type tissue slice glassivation refrigeration carrier and refrigeration method for ovary tissue

A technology of vitrification and freezing carriers, applied in biochemical equipment and methods, tissue culture, preservation of human or animal bodies, etc., can solve the problems of increased cytotoxicity, limited application, and reduced freezing rate, etc., to achieve good preservation, Improved cooling rate and easy operation

Active Publication Date: 2008-12-03
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vitrification is an ultra-rapid freezing method proposed in recent years. It takes less time than programmed freezing, is convenient and cheap, and has no ice crystal damage. Studies believe that it is more suitable for cryopreservation of tissues or organs with different cell components, but vitrification has disadvantages. Yes: The concentration of cryoprotectant required is high, resulting in increased cytotoxicity, which limits its application in the preservation of human tissues and organs
These methods either cannot fully expose the tissue to liquid nitrogen, or the cryoprotectant residue on the tissue surface will reduce the freezing rate, especially when multiple samples are frozen at the same time, it will increase the difficulty of operation, and it is difficult to ensure that each sample is dehydrated and frozen under the same conditions

Method used

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  • Aculeus type tissue slice glassivation refrigeration carrier and refrigeration method for ovary tissue
  • Aculeus type tissue slice glassivation refrigeration carrier and refrigeration method for ovary tissue
  • Aculeus type tissue slice glassivation refrigeration carrier and refrigeration method for ovary tissue

Examples

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Effect test

Embodiment 1

[0040] In this embodiment, the structure of the needle-punched tissue sheet vitrification carrier is as follows: figure 1 As shown, it consists of a cryopreservation tube 1 and an acupuncture device. The cryopreservation tube 1 is a round tube with one end closed and one end open, made of PE (polyethylene) material, with a length of 40 mm and an inner diameter of 6 mm. The structure of the acupuncture device is as Figure 8 , Figure 9 As shown, it is made up of a needle 7 and a needle 8 for piercing tissue pieces. The lower section of the needle 7 is a conical shape that matches the inner shape and size of the opening end of the cryotube 1, and its upper section is a rectangular plate (vitrification cooling). Operation clamping position), needle 8 is two, made of stainless steel, its diameter 0.14mm, length 30mm, is installed in the lower end of needle head 7 apart from certain spacing. The combination of cryopreservation tube 1 and acupuncture device is as follows: figur...

Embodiment 2

[0042] In this embodiment, the structure of the needle-punched tissue sheet vitrification carrier is as follows: figure 2 As shown, it consists of a cryopreservation tube 1 and an acupuncture device. The cryopreservation tube 1 is a round tube with one end closed and one end open, made of PE (polyethylene) material, with a length of 50 mm and an inner diameter of 6 mm. A partition 2 is arranged in the cryo-preservation tube, and the distance between the partition and the closed end is L1 10mm, and the distance L2 between the open end is 40mm, the space surrounded by the partition 2, the closed end and the tube wall between them is equipped with a decompression filler cotton rope 4, and the closure of the partition 2 and the cryopreservation tube 1 The end wall is provided with a decompression hole 3 with a diameter of 1.5mm. The structure of the acupuncture device is as image 3 As shown, it consists of a long handle 5, a short handle 6, a needle head 7 and a needle 8 for p...

Embodiment 3

[0044] In this embodiment, the vitrification carrier of needle-punched tissue pieces consists of a cryopreservation tube 1 and a needle-punching device. Cryopreservation tube 1 is identical with embodiment 1; The structure of acupuncture device is as follows Figure 4 As shown, it consists of a long handle 5, a short handle 6, a needle 7, and a needle 8 for piercing tissue sheets. The long handle 5 and the short handle 6 are two components. The lower section of the long handle 5 is a cone, and the short handle 6 The upper section of the handle is provided with a conical hole matching the long handle 5, and the long handle 5 and the short handle 6 are plug-in connections. The lower part of the needle 7 is conical with the internal shape and size of the cryopreservation tube 1 open end, its upper end is connected with the short handle 6, and the needle 8 is one, made of stainless steel, with a diameter of 0.16mm and a length of 35mm. After assembly, the needle 8 is inserted int...

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Abstract

An acupuncture-type tissue slice vitrification freezing carrier is composed of a freezing tube with one end being closed and the other end being open, and an acupuncture device. The acupuncture device comprises a needle head and a needle disposed on the needle head and used for threading tissue slices. A section matching with the internal shape and size of the open end of the freezing tube is disposed on the needle head. After the acupuncture device and the freezing tube are combined, the needle is positioned in the freezing tube, and the needle head is adopted as a tube plug of the freezing tube to seal the open end of the freezing tube. A plurality of tissue slices (such as ovary tissue slices and liver tissue slices) can be threaded in a row on the needle at intervals by the carrier so as to be subjected to each operation of the vitrification freezing and storage, and a plurality of sample tissues can be subjected to the vitrification freezing and storage simultaneously, with simple operation and high efficiency, so that the sample tissues can be dehydrated and refrigerated under the same conditions. The storage of the ovary multicellular ingredients (such as the storage of interstitial cells and extracellular matrix ingredients) is superior to the programmed slow freezing and the carrier-free vitrification freezing method.

Description

technical field [0001] The invention belongs to the field of vitrification cryopreservation, in particular to a vitrification freezer and freezing method for organs or tissues such as ovaries and livers. Background technique [0002] With the advancement of cryopreservation technology, freezing technology is not limited to freezing cells, gametes or embryos. The cryopreservation of tissues and organs is the development trend of cryogenic technology. Vitrification is an ultra-rapid freezing method proposed in recent years. It takes less time than programmed freezing, is convenient and cheap, and has no ice crystal damage. Studies believe that it is more suitable for cryopreservation of tissues or organs with different cell components, but vitrification has disadvantages. Yes: The concentration of cryoprotectant required is high, resulting in increased cytotoxicity, which limits its application in the preservation of human tissues and organs. Improving the freezing carrier o...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12N5/08A01N1/02
Inventor 李尚为汪燕
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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