Method for extracting stem-possessing plant leaf fibrovascular bundle juice

Abstract

The invention relates to the botany field, in particular to a method for extracting juice in a vascular bundle of a plant leaf with stems, comprising the following steps of: circularly cutting along 0.5 to 1cm of the outer margin of the leaf by a clean and sharp blade to open the vascular bundle of the leaf margin; retaining 3 to 8cm of the stem, and removing the superfluous stems by the blade; semi-circularly cutting the position which is between 0.5 and 1cm from the end part of the stem, and removing the sieve vessel part; putting the processed plant leaf into a depression box, leaving the stem outside the depression box, pressurizing the depression box to enable juice in the sieve vessel of the leaf to flow out from a cut of the stem, and collecting the juice flowing out from the cut of the sieve vessel. The method has the advantages of wide application range, large acquisition of samples, no pollution of samples and more accurate test result.

Description

A method for extracting vascular bundle juice of stalked plant leaves technical field The invention relates to the field of botany, in particular to a method for extracting vascular bundle juice from leaves of stalked plants. Background technique Piercing-sucking mouthparts pests use their stylets to insert into the vascular bundles of the host plant sieve tubes to suck sap to obtain nutrients (Gerling, D. and M. Lindenbaum. Host-plant related behavior of Bemisiatabaci. WPRS Bull., 1991, 14: 83-88.) (Liu Guangjie, WilkinsRM, SaxenaRC. Utilization of carbohydrates in different insect-resistant rice plants by white-backed planthoppers. Acta Entomological Sinica, 1995, 38(4): 421-427), therefore, in order to accurately analyze host nutrition and sucking mouth Therefore, it is necessary to obtain the plant vascular bundle juice accurately. The traditional method of extracting plant vascular bundle juice mainly uses the "aphid kissing needle method", that is, using an aphid t...

AI Technical Summary

Problems solved by technology

In order to overcome the limitations of the existing methods for extracting plant vascular bundle juice, the shortcomings of easy pollution and large errors, the present invention provides a method for directly obtaining the vascular bundle juice of stalked plant leaves by the pressure method, which effectively solves the problem