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Peptide medicaments for accelerating medulla hematopoiesis cell proliferation

A technology of bone marrow hematopoietic cells and drugs, applied in the field of biotechnology and medicine, can solve problems such as limited sources, achieve normal cell structure, solve diseases, and promote the effect of bone marrow hematopoietic cell proliferation

Inactive Publication Date: 2008-09-24
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The choice of treatment for anemia needs to be determined according to the cause of the anemia. For acute hemorrhagic anemia, the most common blood transfusion can be used to replenish blood. However, for chronic anemia and anemia caused by bone marrow hematopoietic dysfunction, simple blood transfusion can not be enough. fixed
[0003] Bone marrow transplantation is one of the currently used methods of enriching blood. It is generally used in the treatment of patients with aplastic anemia or leukemia. However, because the hematopoietic stem cells used for bone marrow transplantation need to be matched, and the source is extremely limited, it is also limited. For the application of this treatment method, the source of stem cells is a crucial issue. Therefore, the emergence of a method capable of cloning stem cells by itself is expected.

Method used

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  • Peptide medicaments for accelerating medulla hematopoiesis cell proliferation
  • Peptide medicaments for accelerating medulla hematopoiesis cell proliferation
  • Peptide medicaments for accelerating medulla hematopoiesis cell proliferation

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preparation example Construction

[0020] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to the inert solid phase carrier one by one, and then use strong acid to cleave the peptide chain from the carrier, and remove the side chain protection at the same time.

[0021] The amino acid main sequence of the short peptide is a main sequence with fixed amino acid positions, and the corresponding amino acids are selected and arranged at the interval positions. For example, there are two kinds of Phe or Ile amino acids at the J position; these two kinds of amino acids can be placed in a single At the position of J in , no matter which one is selected, the short peptide still has its original biological activity, that is, it promotes the proliferation of bone marrow hematopoietic cells, and t...

Embodiment 1

[0038] Promoting bone marrow nucleated cell proliferation in patients with aplastic anemia (Ile-Asn-Asp-Leu-Gly-Leu-Lys)

[0039] Bone marrow cells from human aplastic anemia patients were isolated from mononuclear cells by lymphocyte separation medium, and planted into 96-well cell plates. 5 Cell density per well was planted, and the short peptides involved in the present invention were added to the cell culture plate according to different concentrations, and an equal volume of PBS was used as the control group. The experimental results showed that the bone marrow nucleated cells were under the action of short peptide drugs. , the rate of promoting proliferation reached 175% in 3 days.

Embodiment 2

[0041] Morphological examination after promoting the proliferation of bone marrow nucleated cells (synthesize the short peptide fragments involved in the present invention, truncating 4 or 5 amino acids in the sequence: Asn-Asp-Leu-Gly- or Asp-Leu-Gly-Leu-Lys )

[0042] Bone marrow nucleated cells were cultured with DMEM medium containing 10% fetal calf serum, and the culture medium was 10 6 After the cell density per well was planted into a 24-well cell plate, the short peptides involved in the present invention were immediately added to the cell culture plate at different concentrations, and an equal volume of PBS was used as the control group. After 24 hours, the cells were centrifuged and removed. Supernatant, pre-fixed cell pellets with 3% glutaraldehyde-1.5% paraformaldehyde for 1 day (4°C), post-fixed with 1% osmic acid-1.5% potassium ferrocyanide for 1.5h, rinsed with PBS; 70% alcohol Block staining with saturated uranyl acetate dye solution, alcohol-acetone gradient ...

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Abstract

The present invention provides a peptide drug that can promote the proliferation of marrow hematopoietic cells. The main amino acid sequence of the short peptide fragments, analogs, derivants and variants of the short peptide is larger than or equal to 70 percent, equal to and larger than or equal to 90 percent similar to the main amino acid sequence of the short peptide. The short peptide, nucleotides, short peptide fragments, short peptide analogs, short peptide derivants and short peptide variants are used for the preparation of drugs for treating manifold marrow hematopoietic cell defection diseases, congenital anaemia, aplastic anemia and various hemorrhagic anemia diseases and can be applied to the bone marrow transplantation of leukemia patients, etc., that is, the peptide drug of the present invention can treat pancytopenia caused by hemopoietic stem cell defects.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a peptide medicine for promoting the proliferation of bone marrow hematopoietic cells. Background technique [0002] The choice of treatment for anemia needs to be determined according to the cause of the anemia. For acute hemorrhagic anemia, the most common blood transfusion can be used to replenish blood. However, for chronic anemia and anemia caused by bone marrow hematopoietic dysfunction, simple blood transfusion can not be enough. solved. [0003] Bone marrow transplantation is one of the currently used methods of enriching blood. It is generally used in the treatment of patients with aplastic anemia or leukemia. However, because the hematopoietic stem cells used for bone marrow transplantation need to be matched, and the source is extremely limited, it is also limited. For the application of this treatment method, the source of stem cells is a cru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/00C12N15/11C07H21/04A61K38/08A61K31/7088A61P7/06C12Q1/68G01N33/68
Inventor 李昊饶平凡
Owner FUZHOU UNIV
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