Preparation of artificial skin containing hair follicle and artificial skin prepared by the same
A technology of artificial skin and hair follicles, applied in the field of biomedicine, can solve the problems of artificial skin antigen weakening and skin source difficulties, and achieve the effects of solving skin source difficulties, promoting normal healing, and reasonable design
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Embodiment 1
[0031] (1) Preparation of hair follicle epithelial cells: Isolate human scalp hair follicles, add a certain concentration of 0.5% dispase enzyme (dispase, Sigma), digest for 12 to 18 hours at 4°C, transfer the hair follicles into D-Hanks buffered salt solution, and rinse Remove the separating enzyme, squeeze the hair shaft from the hair follicle, digest it with 0.15% trypsin plus 0.02% EDTA (Sigma) into individual hair follicle epithelial cells, centrifuge to remove trypsin, use serum-free keratinocyte culture medium, containing epidermal growth factor (EGF, Sigma-aldrich company) 10ng / mL, hydrocortisone 0.4μg / mL, insulin 5μg / mL, culture for use.
[0032] (2) Hair papilla cell culture: The human hair follicles from which the hair shaft has been removed are digested with 0.1-0.5% collagenase VI at 37°C for 2-6 hours. The dermal sheath surrounding the hair follicle is digested into single cells and the hair papilla inside it When the digestion has not yet started, stop the digestio...
Embodiment 2
[0036] The subcultured dermal papilla cells (4 / ml. Other technical solutions are as in Example 1. After 6 weeks of culture, H.E. staining was performed, and the formation of hair follicle-like structures was seen.
Embodiment 3
[0038] The subcultured dermal papilla cells (5 / ml. Other technical solutions are as in Example 1. After 6 weeks of culture, H.E. staining was performed, and the formation of hair follicle-like structures was seen.
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