Golden leaf carex shoot-tip culture quick propagation method
A technology of Carex aureus and shoot tips, which is applied in the field of rapid propagation of Carex aureus shoot tips, can solve the problems of inability to realize large-scale industrial production, unstable properties of golden leaves of tissue culture seedlings, and different degrees of difficulty, and achieve the goal of reducing group production. Differentiation of seedlings, good applicability, and the effect of preventing inoculation contamination
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Embodiment 1
[0032] 1) The preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter are:
[0033] (1) Basic medium: select MS medium, agar 7g / L, pH5.8;
[0034] (2) Induction medium: MS+KT 1.0mg / L+IAA 0.1mg / L+sucrose 30g / L;
[0035] (3) Proliferation medium: MS+KT 0.5mg / L+IAA 0.1mg / L+white sugar 30g / L;
[0036] (4) Strong seedling medium: MS+KT0.25mg / L+IAA0.05mg / L+sugar 30g / L;
[0037] (5) Rooting medium: 1 / 2MS+NAA0.2mg / L+IAA 0.1mg / L+sugar 20g / L;
[0038] 2) Selection and sterilization of explants: During the vigorous growth season of Carex golden leaf tillering, at 9-10 o'clock in the morning on a sunny day, select good plants without disease, take tiller buds with a height of about 5-10 cm, and remove the upper slender leaves and the root system at the bottom are sorted into a section with a growth point and a length of about 1.5 cm as an explant, which is sterilized for subs...
Embodiment 2
[0048] In this example, basic medium: select MS medium, agar 8g / L, pH5.6; induction medium: MS+KT 0.5mg / L+IAA0.05mg / L+ sucrose 30g / L; proliferation medium: MS+ KT 0.1mg / L+IAA0.05mg / L+sugar 30g / L; strong seedling medium: MS+KT 0.1mg / L+IAA 0.05mg / L+sugar 30g / L; rooting medium: 1 / 2MS+NAA0.1mg / L+IAA0.05mg / L+white sugar 20g / L.
[0049] All the other steps and conditions are the same as in Example 1.
Embodiment 3
[0051] In this example, basic medium: select MS medium, agar 9g / L, pH5.7; induction medium: MS+KT2.0mg / L+IAA 0.2mg / L+ sucrose 30g / L; proliferation medium: MS+ KT 1.0mg / L+IAA0.2mg / L+sugar 30g / L; strong seedling medium: MS+KT 0.5mg / L+IAA 0.2mg / L+sugar 30g / L; rooting medium: 1 / 2MS+NAA0.5mg / L+IAA0.2mg / L+white sugar 20g / L.
[0052] All the other steps and conditions are the same as in Example 1.
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