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Cultivation method of rhizoma corydalis stem tuber

The technology of dark cultivation and medium is applied in the field of cultivation method of Corydalis Corydalis tuber, which can solve the problems of low reproduction coefficient, hinder the promotion of improved varieties, and mixed varieties of Corydalis Corydalis, and achieve the effects of rapid provision, speeding up the promotion of improved varieties, and improving planting purity.

Inactive Publication Date: 2010-06-09
ZHEJIANG UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In terms of production, the species of Corydalis are mixed seriously, resulting in a low degree of standardization of the cultivation technology system; for a long time, the asexual reproduction method of tuber breeding has been used for reproduction, and the reproduction coefficient is low, which seriously hinders the promotion of improved varieties.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, a kind of cultivation method of Corydalis tuber, carries out following steps successively:

[0019] 1), put the Corydalis tuber that has no disease spots, no galls and is strong and full into a biochemical incubator for germination until at least one bud eye that germinates from the Corydalis tuber grows into a small bud with a height of 0.3cm. The culture conditions are: Cultivate in the dark at 26-28°C.

[0020] 2), above-mentioned corydalis tuber with bud is cut into the tuber with bud that growth * width * height is 0.2cm * 0.5cm * 0.1cm; After the above-mentioned tuber with bud is routinely sterilized, it is inoculated on the MS medium containing activated carbon to carry out Culture; culture conditions: 16 hours of light, light intensity 10 μmol m -2 .s -1 , the temperature is 26-28°C; 8 hours of dark cultivation, the temperature is 20-22°C; the above-mentioned light and dark cultivation are carried out alternately.

[0021] The MS medium containi...

Embodiment 2

[0029] Embodiment 2, a kind of cultivation method of Corydalis tuber, carries out following steps successively:

[0030] 1) Put the Corydalis tubers that are free from disease spots, galls, and strong and plump into a biochemical incubator for germination until all bud eyes are completely germinated (that is, small buds grow); the culture conditions are: dark culture at 26-28°C .

[0031] 2), above-mentioned corydalis tuber with bud is cut into the tuber with bud that growth * width * height is 0.3cm * 0.4cm * 0.2cm; After the above-mentioned tuber with bud is routinely sterilized, it is inoculated on the MS medium containing activated carbon to carry out Culture; culture conditions: 16 hours of light, light intensity 20μmol m -2 .s -1 , the temperature is 26-28°C; 8 hours of dark cultivation, the temperature is 20-22°C; the above-mentioned light and dark cultivation are carried out alternately.

[0032] The MS medium containing activated carbon is MS+2.5% sugar+0.5% agar+0...

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Abstract

The invention discloses the implantation method of the rhizoma corydalis tuber, which includes the following steps: (1) imbedding the strong and plump rhizoma corydalis tuber of non-disease spot and non-cecidum in the biochemical incubator and sprouting; (2) cutting the rhizoma corydalis tuber with buds into the required tubers with the buds, inoculating to the MS culture medium which contains theactive carbon and culturing after regular sterilization; (3) when the buds on the tuber grow into the seedlings, cutting the seedlings; (4) incubating the seedlings to the tuber inducing culture medium and culturing; (5) when the seedlings grow into the small quasi-spherical tubers, incubating the small tubers to the tuber proliferating culture medium and culturing the small tubers to required standard; (6) When the obtained buds on the tuber again grow into seedlings in Step (3), cutting the seedlings and sequentially repeating Steps (4) and (5). Through the method, a great quantity of rhizoma corydalis tubers can be harvested.

Description

technical field [0001] The invention relates to a method for cultivating corydalis tubers. Background technique [0002] In terms of production, the species of Corydalis are mixed seriously, resulting in a low degree of standardization of the cultivation technology system; and the asexual reproduction method of tuber breeding has been used for a long time, and the reproduction coefficient is low, which seriously hinders the promotion of improved varieties. According to the production survey, the field tuber propagation coefficient of Corydalis is generally about 10 times, and the degree of mixed varieties in the field is as high as 40%, which seriously affects the large-scale application of excellent varieties and the consistency and stability of medicinal material quality. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for cultivating Corydalis tubers, which can obtain a large number of Corydalis tuber...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01C1/00
Inventor 毛碧增冯利张国芳孙丽
Owner ZHEJIANG UNIV
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