Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding

Abstract

The invention discloses a quasi-physiological pulsatile flow environment arterial blood vessel tissue engineering reactor; the reactor includes a liquid storage bottle, a pulsation source, a resistance regulator, a compliance regulator and a blood vessel tissue dynamic culture cavity and is controlled by an industrial control computer; the reactor overcomes the shortcomings that the commonly used arterial blood vessel tissue engineering reactor in the tissue engineering filed can not simulate the blood flow compliance of the arterial blood vessel, flow inertia, flow resistance and other resistance characteristics and can not provide the quasi blood pulsatile flow environment in the artery; the reactor has the following functions that: a. the reactor is used for culturing the tissue engineering arterial blood vessel; b. the reactor is used for simulating the pulsating frequency, pulse waveform, secondary wave, amplitude and time phase of the pulsatile flow of the different arterial blood vessel under the physiological state, simulating the hemodynamics environment of high vessel inner pressure and high shear stress of the arterial blood vessel under the hypertension and other pathological states and simulating the hemodynamics status of the arterial blood vessel during the low shear stress.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more particularly relates to a vascular tissue engineering reactor. Background technique [0002] In the field of tissue engineering, tissue engineering bioreactor is an important technical means to study the influence of different environmental factors (physical and biochemical factors) on the three-dimensional functional culture of specific cells and tissues. Automation, online monitoring, etc.), making tissue engineering a key link in the process of entering standard industrial scale production and clinical application from the laboratory. Therefore, targeted research and development of advanced bioreactors for tissue engineering is of great significance to the development of tissue engineering (Martin, et al., 2004; Portner, R., et al., 2005). [0003] So far, autografts, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels have...

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Problems solved by technology

The above-mentioned reactor basically uses a peristaltic pump or a peristaltic pump plus a stepping motor as the pulsation source, and because the pulse wave simulation optimization design of the fluid circuit is not done, the simulation of flow impedance, compliance, resistance, and flow inertia is not carefully considered. As a result, existing reactors cannot provide a near-physiological flow environment similar to the pulsating flow state of blood in arteries

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