Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Side sequence of exogenous insert of transgene paddy strain Bt Shanyou 63

A technology of transgenic rice and flanking sequences, which is applied in the fields of genetic engineering, plant genetic improvement, and microbial determination/inspection, etc.

Inactive Publication Date: 2008-08-13
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
View PDF0 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the analysis of existing patents and documents, it is found that there are no articles or patent reports on the flanking sequence of the foreign insert of the transgenic rice line Bt Shanyou 63

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Side sequence of exogenous insert of transgene paddy strain Bt Shanyou 63
  • Side sequence of exogenous insert of transgene paddy strain Bt Shanyou 63
  • Side sequence of exogenous insert of transgene paddy strain Bt Shanyou 63

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cloning of the Side Sequence of the Foreign Insertion Vector of the Transgenic Rice Line Bt Shanyou 63

[0032] 1. Experimental materials

[0033] 1. Plant material

[0034]Transgenic rice: Transgenic rice line Bt Shanyou 63.

[0035] Conventional rice: Shanyou 63.

[0036] 2. Enzymes and reagents

[0037] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000Marker were purchased from Dalian Bao Biological Engineering Co., Ltd. Other biochemical reagents are imported subpackages or domestic analytical pure. Primers and degenerate primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0038] 3. Experimental equipment

[0039] PCR instrument: Eppendorf Mastercycle gradient (Eppendorf co.)

[0040] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0041] DNA sequencer (Applied Biosystem377 automatic fluorescent sequence analyzer)

[0042] DNA electrophoresis...

Embodiment 2

[0141] Qualitative PCR detection method based on the flanking sequence of the exogenous insertion vector of the transgenic rice line Bt Shanyou 63

[0142] 1. Experimental materials

[0143] 1. Plant material

[0144] Transgenic rice: Transgenic rice line Bt Shanyou 63.

[0145] Conventional rice: Shanyou 63.

[0146] 2. Enzymes and reagents

[0147] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000Marker were purchased from Dalian Bao Biological Engineering Co., Ltd.

[0148] Other biochemical reagents are imported subpackages or domestic analytical pure. Primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0149] 3. Experimental equipment

[0150] PCR instrument: Eppendorf Mastercycle gradient (Eppendorf co.)

[0151] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0152] DNA electrophoresis analysis system: Kodak EDAS 290 (Kodak co.)

[0153] Other in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a side sequence of exogenous interpose fragments of transgene paddy rice line Bt SHANYOU 63. its 5' side sequence is shown as SEQ ID NO1 and its 3' side sequence is shown as SEQ ID NO2. The line specificity determine the nature and definite quantity PCR detection of sensitive idiosyncratic transgene paddy rice line Bt SHANYOU 63 is constructed by using side sequence as aim DNA amplification fragments.

Description

technical field [0001] The present invention relates to a gene sequence in the field of biotechnology. Specifically, it relates to a flanking sequence of an exogenous insertion fragment of a transgenic rice line Bt Shanyou 63. Background technique [0002] Rice is one of the most important food crops in my country. With the extensive research and application of transgenic technology in rice, several transgenic rice strains have been released to the environment and applied for commercial planting. Strain-specific detection of transgenic plants is an important technical basis for the supervision and management of transgenic plants to ensure their healthy development. The flanking sequence of the exogenous insert in a transgenic plant is one of the most important molecular characteristics of a transgenic plant line. Therefore, the flanking sequence of the exogenous insert is an important technical data for establishing a specific detection method for a transgenic plant line. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12Q1/68
Inventor 谢家建王锡锋彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com