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Technique for producing hirudin by using poecilobdella manillensis saliva as raw material

A production process and a technology of hirudo are applied in the field of production technology for extracting hirudin, and can solve the problems of low yield and high activity of hirudin

Inactive Publication Date: 2008-07-30
GUANGDONG OCEAN UNIVERSITY
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Problems solved by technology

The hirudin obtained by this method has high activity and good medicinal effect, and the method of extracting hirudin is simple, but the yield is extremely low

Method used

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  • Technique for producing hirudin by using poecilobdella manillensis saliva as raw material

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Embodiment Construction

[0007] The present invention realizes through the following measures:

[0008] After thawing and liquefying the frozen saliva, add an equal volume of 0.15 mol / L sodium chloride solution and mix well. Stir at 240r / min for 2 hours, boil and cool to room temperature rapidly. Filter through a 120-mesh nylon mesh and collect the filtrate. The solid matter is added to 0.15mol / L sodium chloride solution with 1.5 times the volume of the saliva of the saliva to make a uniform suspension. Stir at 180r / min for 1 hour, filter with 150 mesh nylon mesh. The filtrate was collected, and the solid matter was extracted twice by repeating the above operation. Discard the solids, combine the three filtrates, boil and cool to room temperature quickly, and combine all the filtrates to refrigerate overnight. The refrigerated filtrate was filtered successively with 150-mesh and 300-mesh nylon mesh, the solids were discarded, and the filtrate was collected. Add Lyphgel at a ratio of 50:1 accordin...

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Abstract

The invention discloses a hirudin manufacturing technique with the saliva of poecilobdella manillensis as the raw material, which belongs to the traditional Chinese medicine pharmacy field, relates to extraction of effective elements of animals, and aims at solving the problem that the traditional hirudin extraction technique is difficult to realize industrialized production. The technique adopts the saliva of poecilobdella manillensis to extract hirudin, and industrialized production can be realized. The saliva of poecilobdella manillensis is extracted through normal saline, the extracting solution is boiled and filtered, and the filter liquor is obtained. The solids is extracted through normal saline, and filtered, and the filter liquor is obtained. All the filter liquor produced in the two steps is combined together, Lyphgel is added to treat for twice, filtering is performed, and the filter liquor is removed. The solids is extracted through normal saline and filtered. After being boiled, the filter liquor is cooled down rapidly, and refrigerated over night. Centrifugation is performed, the supernatant is taken, and the filter liquor is frozen and dried into powder after decompression and condensation. The manufacturing technique is simple, the product activity is high, and has very strong thrombus dissolution function.

Description

technical field [0001] The process relates to the field of traditional Chinese medicine pharmacy, and specifically relates to a production process for extracting hirudin from the saliva of Hirudo phenatus as a raw material. Background technique [0002] my country has abundant leech resources that can be utilized. Leech is a commonly used drug for promoting blood circulation and removing blood stasis in traditional Chinese medicine, and many Chinese patent medicines based on leeches are popular in the market. But this kind of medicine is inconvenient to use, and the curative effect is not obvious. [0003] The leech involved in the process is Hirudo phenanthae, a blood-sucking leech of the family Hirudoceae, which is widely distributed in Hainan, Guangdong, Guangxi, Fujian, Yunnan and other places in my country. The application of the phenanthrene provides effective drugs for the prevention and treatment of cardiovascular diseases. The saliva used in this process refers t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K2/00
Inventor 方富永宋文东苗艳丽李思东彭少伟范润珍黄甫谷长生林宏图覃亮
Owner GUANGDONG OCEAN UNIVERSITY
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