Lactose enzyme and common immobilization method of glucose isomerase

Abstract

A co-immobilization method for lactase and glucose isomerase belongs to the food biology technology area. The invention uses gelatine, sodium polymannuronate and / or carrageenan as the carrier; the first embedding and after cross linking process is applied; or the nylon net is used as the carrier; the first cross linking and after covalent combination method is used for the co-immobilization of lactase and glucose isomerase. The distinct characteristics of the invention are that the co-immobilization of lactase and glucose isomerase can not only realize recycle utilization of the enzyme which saves the economic cost and simplifies the downstream separation steps, but also exert the cooperation function between two enzymes which improves the reaction efficiency. The invention has a novel idea, a simple process and a strong practicality. The obtained lactase and glucose isomerase can be used for the transformation of lactose for the preparation of lactulose. The invention has good application prospect.

Description

A co-immobilization method of lactase and glucose isomerase technical field The invention relates to a co-immobilization method of lactase and glucose isomerase, in particular to an embedding-crosslinking method using gelatin, sodium alginate or / and carrageenan as a carrier; or a crosslinking method using nylon mesh as a carrier -Covalent method, co-immobilization of lactase and glucose isomerase, belonging to the field of food biotechnology. Background technique The use of free enzymes in large-scale industrial production will not only increase the production cost, but also cause the quality of the product to be low due to the addition of extra protein. After the enzyme is immobilized, it can not only realize continuous operation and repeated recycling, but also simplify the subsequent separation steps. The physical and chemical properties of the immobilized enzyme will change to a certain extent, and the stability to temperature and pH will be enhanced, which has great ...

AI Technical Summary

Problems solved by technology

At present, the industrial production of lactulose mainly adopts the chemical catalysis method, but the reaction conditions of this method are severe, the product obtained is dark in color, there are many by-products, and the separation of the catalyst is difficult, which brings difficulties to the further purification of the syrup and the preparation of crystallization.

However, the preparation of lactulose by enzymatic method has strong specificity, less by-products and light color of the product, but the cost is higher

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