Matrix auxiliary laser decomposition-absorption-particle bundle collision decomposition flying time mass spectrograph

Abstract

A base material assisting laser desorption-particle beam collision dissociation time-of-flight mass spectrometer relates to a mass spectrograph and provides a time-of-flight mass spectrometer obviously decreasing noise disturbance and improving mass resolution. The invention is provided with a cavity, a sample introduction probe rod, a preposition and a postposition mesh units, a particle beam generator, a multi-polar rod, a particle beam detector, a microchannel plate electron multiplier, a laser and a focusing lens. The sample introduction probe rod, the preposition mesh unit, the postposition mesh unit, the particle beam generator, the multi-polar rod, the particle beam detector, the microchannel electron multiplier are arranged inside the cavity in turn. The cavity and the sample introduction probe rod are coaxial. The axle wire of the postposition mesh unit is coaxial with the particle beam generator, the multi-polar rod and the particle beam detector. The cavity is provided with a quartz window for laser in, a vacuum opening for extraction and a valve for sample introduction probe rod in and out on the wall. The sample introduction probe rod is provided with a sampling fixing component on the top end. The laser and the focusing lens are arranged outside the cavity.

Description

technical field [0001] The invention relates to a mass spectrometer, in particular to a matrix-assisted laser desorption-particle beam collision dissociation time-of-flight mass spectrometer. Background technique [0002] Currently, techniques commonly used in the analysis of biomacromolecules include gel electrophoresis and mass spectrometry. Mass spectrometry has the characteristics of high resolution, high precision and high sensitivity. Among them, matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have been widely used in the analysis of biological macromolecules. For biological macromolecules with millions of Daltons, although MALDI technology can ionize them, the signal value is very weak and difficult to detect. In an ESI source, biomacromolecules exist in the form of multiple charges, and the resulting spectra are very complex and difficult to interpret. Moreover, most mass analyzers, such as quadrupoles, sector fields, and ion ...

AI Technical Summary

Problems solved by technology

Considering the limitations of vacuum degree, instruments and other factors, it is impossible to achieve up to 5×10 6 Accelerating voltage of V

Therefore, the method of characterizing supermacromolecules by measuring molecular weight is not feasible

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