Kit for fast detecting multiple drug resistance of mycobacteriumtuberculosis

Abstract

The invention belongs to the biomedical test field, and relates to a microbial molecular biological detection method, in particular to a kit for rapidly testing the multi-drug resistance of mycobacterium tuberculosis. The kit comprises a multiple PCR system simultaneously amplifying four kinds of drug resistance related genes of noddle, and a probe hybrid system simultaneously detecting corresponding point mutation in the drug resistance related genes. The kit can simultaneously detect the mutation of a plurality of drug resistance related genes of noddle through one-time operation, thereby being rapid and time saving, and only needing one working day for the entire process. The kit which has relatively low cost is not required to be provided with an expensive chip reading machine, thereby being easy for implementation and promotion in grass-root medical institutions.

Description

technical field [0001] The invention belongs to the field of biomedical testing, and relates to a detection method of microbial molecular biology, in particular to a kit for rapidly detecting multiple drug resistance of Mycobacterium tuberculosis. Background technique [0002] In recent years, tuberculosis has shown a clear upward trend in the world. In addition to HIV infection, MDR-TB has become one of the main reasons for the global TB resurgence. The main treatment strategy for multidrug-resistant tuberculosis is to avoid the use of anti-tuberculosis drugs resistant to tuberculosis, which requires rapid acquisition of drug resistance data, but the current traditional methods of culture and drug susceptibility testing often require one month to obtain resistance Drug resistance data, if the culture is negative, the drug resistance data cannot be obtained at all. It is an urgent need for tuberculosis prevention and control to establish a method for rapid detection of tub...

AI Technical Summary

Problems solved by technology

However, although gene chip technology is sensitive, accurate, and fast, it is too expensive, and its clinical application is still controversial.

And there is no mature product at present; existing studies have used the allelic multiplex PCR method to simultaneously identify rifampicin and isoniazid resistance (①Jin Jialin, Zhang Wenhong, etc., Chinese Journal of Tuberculosis and Respiratory Science, 2005, 28(9): 623- 625; Jin Jialin, Zhang Wenhong, etc., Chinese Journal of Infectious Diseases, 2005, 23(3): 146-149)

However, the method can only detect limited drug-resistant genes, and can only cover 2 drugs, rifampicin and isoniazid, and the sensitivity is not high; it is reported that the reverse hybridization technique is used to detect drug-resistant genes (Pang Maoyin, Zhang Wen Hong, Hu Zhongyi, et al. Chinese Journal of Infectious Diseases 2004; 22(4): 234-236; Liu Yang, et al. Chinese Journal of Laboratory Medicine 2005; 28(4): 394-396)

However, this technology can only be used to detect one gene at present and cannot detect multiple genes at the same time

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