Reagent kit detecting pulmonary cancer susceptibility through CYP2E1 gene

A kit and susceptibility technology, which is applied in the field of kits for detecting susceptibility to lung cancer, and can solve problems such as the decrease of CYP2E1 transcription level

Inactive Publication Date: 2008-03-12
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The transcription level of Rsa I RFLP affects the transcriptional regulation of genes, and affects the expression of CYP2E1 from the mRNA level, that is, the c2 allele reduces the transcription level of CYP2E1

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Use of detection kits

[0024] Step 1: Extraction of DNA template

[0025] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0026] Step 2: Real-time quantitative PCR reaction

[0027] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0028] Sense primer: 5'-ACAAGTGATTTGGCTGGATT-3' (SEQ ID NO: 2) with a Tm value of 56°C

[0029] Antisense primer: 5'-AATGAAGAGAATGTTTTTCATT-3' (SEQ ID NO: 3) with a Tm value of 54°C

[0030] Fluorescent probe: 5'-AGATTCATTGTTAATAATAAAAGTATAAA-3' (SEQ ID NO: 4) with a Tm value of 64°C

[0031] The fluorescent quantitative PCR reaction system has a total volume of 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, 0.1 μl of 25mM dNTP mixture, and 0.6 μl of 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA ...

Embodiment 2

[0035] Example 2. Services that guide people to proactively prevent lung cancer

[0036] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0037] Step 1: DNA Extraction

[0038] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0039] Step 2: Genotyping Assays

[0040] Using the kit provided by the present invention, the SNP site rs2031920 on the CYP2E1 gene of the tested subject's DNA is detected by fluorescent quantitative PCR, and the genotype of the SNP site is determined.

[0041] Step 3: Instruct people to be proactive in preventing lung cancer

[0042] Through the analysis of the SNP genotype of the tested person, the genotyping test report and...

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PUM

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Abstract

The present invention discloses a reagent kit to detect lung cancer susceptibility, which comprises a specificity tracer and a specificity fluorescence probe to detect No. 176 SNP locus of gene SEQ ID No: 1 of CYP2E1 and a normal assembly for quantitative fluorescence PCR detection. The reagent kit of the present invention forecasts individual lung cancer susceptibility by analyzing individual carrying type of SNP locus gene of on CYP2E1 gene.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer, by detecting the single nucleotide of cytochrome P4502E1 enzyme (cytochrome P450, family2, subfamily E, polypeptide1, CYP2E1) polymorphism (SNP) loci to predict individual susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease progression is related to the biological characteristics of the cells. [0003] For half a century, the morbidity and mortality of lung cancer in all countries in the world have ...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/64C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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