Application of whiterof fungus prepn in improving the continuous cropping performance of soil
A technology for continuous cropping obstacles and soil improvement, which is used in applications, soil conditioning materials, organic fertilizers, etc.
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Embodiment 1
[0025] Embodiment 1, with 100ml white rot fungus spore suspension (10 5 cfu / ml) is diluted with 40 kg of sterilized basal medium and mixed with 50 kg of wood chips sterilized by high-temperature steam to make the water content of the wood chips 50%. Pack one bag per 2kg, culture in a 37°C incubator for 9 days, dry in a 40°C oven for 20 hours, and set aside. Two weeks before sowing, apply white rot fungicide at 400g / m 2 The amount of land is applied to the soil surface at a depth of 10cm, the soil is loosened, and watered to keep it moist.
Embodiment 2
[0026] Embodiment 2, with 200ml white rot fungus spore suspension (10 6 cfu / ml) was diluted with 70 kg of sterilized basal medium and mixed with 80 kg of wood chips sterilized by high-temperature steam to make the water content of the wood chips 50%. Pack one bag per 2kg, culture in a 30°C incubator for 10 days, dry in a 45°C oven for 22 hours, and set aside. Two weeks before sowing, apply white rot fungicide at 500g / m 2 The amount of land is applied to the soil surface at a depth of 20cm, loosened, and watered to keep it moist.
Embodiment 3
[0027] Embodiment 3, with 100ml white rot fungus spore suspension (10 5 cfu / ml) was diluted with 75kg of sterilized basal medium and mixed with 60kg of wood chips sterilized by high-temperature steam to make the water content of the wood chips 60%. Pack one bag per 2kg, culture in a 35°C incubator for 8 days, dry in a 50°C oven for 24 hours, and set aside. Two weeks before sowing, apply white rot fungicide at 450g / m 2 The amount of land is applied to the soil surface at a depth of 30cm, loosened, and watered to keep it moist.
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