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Culture medium with simultaneoulsy enriched salmonella, colibacillus and Staphylococcus aureus

A technology of Escherichia coli, enrichment culture, applied in bacteria, microorganism-based methods, microorganisms, etc., to reduce false negatives, improve accuracy, and improve detection efficiency

Inactive Publication Date: 2007-10-03
陈福生 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the medium that can simultaneously enrich and cultivate Salmonella, Escherichia coli and Staphylococcus aureus commonly found in food

Method used

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Examples

Experimental program
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Embodiment 1

[0017] Embodiment 1: a kind of substratum that can simultaneously enrich and cultivate Salmonella, Escherichia coli, Staphylococcus aureus, its component and content are: peptone 5g, beef extract 1g, sodium chloride 25g, disodium hydrogen phosphate (Na 2 HPO 4 12H 2 (0) 5g, potassium dihydrogen phosphate 0.5g, distilled water 1000mL, pH7.0.

Embodiment 2

[0018] Embodiment 2: a kind of substratum that can enrich and cultivate Salmonella, Escherichia coli, Staphylococcus aureus simultaneously, its component and content are: peptone 10g, beef extract 3g, sodium chloride 50g, disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 9g, potassium dihydrogen phosphate 1.5g, distilled water 1000mL, pH7.2.

Embodiment 3

[0019] Embodiment 3: a kind of substratum that can simultaneously enrich and cultivate Salmonella, Escherichia coli, Staphylococcus aureus, its component and content are: peptone 15g, beef extract 5g, sodium chloride 75g, disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 15g, potassium dihydrogen phosphate 3.0g, distilled water 1000mL, pH7.5.

[0020] The preparation process of the medium of the present invention: Accurately weigh the above-mentioned components, heat and dissolve them in 1000 mL of distilled water, adjust the pH value to 7.2 with 1mol / L NaOH, and autoclave at 121°C for 15 minutes for later use.

[0021] Identification method: inoculate 10 cfu of Salmonella, Escherichia coli, and Staphylococcus aureus into 10 mL of milk sample at the same time, add 90 mL of the medium of the present invention, culture at 37°C for 16 hours, and count using WS plate and Baird-Parker plate. The blue-green colonies on the WS plate are Salmonella, the orange (red) colonies are Esch...

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Abstract

The present invention relates to one kind of culture medium with simultaneously enriched salmonella, colibacillus and Staphylococcus aureus, and belongs to the field of food originated pathogenic bacterium detecting technology. The culture medium with simultaneously enriched salmonella, colibacillus and Staphylococcus aureus consists of peptone 10 g, beef extract 3 g, sodium chloride 50 g, Na2HPO4.12H2O 9 g, potassium dihydrogen phosphate 1.5 g and distilled water 1000 ml, and has pH 7.2.

Description

technical field [0001] The invention relates to a culture medium capable of simultaneously enriching and culturing Salmonella spp., Escherichia coli and Staphylococcus aureus. Background technique [0002] Pathogenic bacteria contaminated in food is one of the main factors causing foodborne diseases. Statistics from the National Foodborne Disease Surveillance Network show that in the past ten years, the number of food poisoning incidents caused by microorganisms and the number of people involved are the largest, among which Vibrio parahemolyticus (Vibrio parahemolyticus), Salmonella (Salmonella spp.) and Escherichia coli are the most common pathogens [1] Therefore, rapid detection and identification of foodborne pathogenic bacteria is very important for timely and effective control of disease transmission and prevention of food poisoning incidents, and some rapid detection technologies have also developed rapidly. However, whether it is a traditional culture method or a ra...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/42C12R1/19C12R1/445
Inventor 陈福生许一平
Owner 陈福生
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