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Complex culture medium for lactobacillus ferment and preparation method thereof

A culture medium and lactobacillus technology, applied in the field of compound culture medium for lactobacillus fermentation and its preparation, can solve problems such as difficult control of quality and production conditions, harmful effects on animal health, complex culture medium formula, etc., and achieve beneficial diversification The development, composition and production process are simple, and the effect of saving energy consumption and equipment cost

Active Publication Date: 2011-06-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current problems are that the formula of the culture medium is complicated, the quality and production conditions are not easy to control, the raw materials are various and the price is high, it is not suitable for large-scale fermentation and cultivation, and too many inorganic salts need to be added, which is harmful to human and animal health. have potentially harmful effects

Method used

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  • Complex culture medium for lactobacillus ferment and preparation method thereof
  • Complex culture medium for lactobacillus ferment and preparation method thereof
  • Complex culture medium for lactobacillus ferment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] The composition of the compound medium for Lactobacillus acidophilus fermentation is:

[0139] 10% licorice decoction filtrate 10000ml

[0140] Polyvalent peptone 100g

[0141] Preparation of compound medium for Lactobacillus acidophilus fermentation:

[0142] (1) Preparation of 10% licorice decoction filtrate (GJY):

[0143] a. Select dry licorice rhizomes or decoction pieces without mildew, wash with water, remove impurities, and dry.

[0144] b. Weigh 1000 g of licorice, and physically pulverize the licorice by mechanical pulverization until a fine powder with an average particle size of 100-150 μm is obtained.

[0145] c. After the licorice fine powder is frozen at a low temperature to the critical point of fragmentation, energy pulverization is carried out by means of airflow pulverization until an ultrafine powder with an average particle size of 5-25 μm is obtained.

[0146] d. The obtained above-mentioned licorice ultrafine powder is fermented and concentrat...

Embodiment 2

[0153] The composition of the compound medium for plantaractobacillus fermentation is:

[0154] 10% licorice decoction filtrate 10000ml

[0155] Polyvalent peptone 200g

[0156] Preparation of compound medium for plantaractobacillus fermentation:

[0157] (1) Preparation of 10% licorice decoction filtrate (GJY):

[0158] a. Select dry licorice rhizomes or decoction pieces without mildew, wash with water, remove impurities, and dry.

[0159] b. Weigh 1000 g of licorice, and physically pulverize the licorice by mechanical pulverization until a fine powder with an average particle size of 100-150 μm is obtained.

[0160] c. After the licorice fine powder is frozen at a low temperature to the critical point of fragmentation, energy pulverization is carried out by means of airflow pulverization until an ultrafine powder with an average particle size of 5-25 μm is obtained.

[0161] d. The obtained above-mentioned licorice ultrafine powder is fermented and concentrated with the ...

Embodiment 3

[0168] The composition of the compound medium for Lactobacillus bulgaricus fermentation is:

[0169] 10% licorice decoction filtrate 10000ml

[0170] Polyvalent peptone 100g

[0171] Liver Infusion Powder 24g

[0172] Preparation of compound medium for Lactobacillus bulgaricus fermentation:

[0173] (1) Preparation of 10% licorice decoction filtrate (GJY):

[0174] a. Select dry licorice rhizomes or decoction pieces without mildew, wash with water, remove impurities, and dry.

[0175] b. Weigh 1500 g of licorice, and physically pulverize the licorice by mechanical pulverization until a fine powder with an average particle size of 100-150 μm is obtained.

[0176] c. After the licorice fine powder is frozen at a low temperature to the critical point of fragmentation, energy pulverization is carried out by means of airflow pulverization until an ultrafine powder with an average particle size of 5-25 μm is obtained.

[0177] d. The obtained above-mentioned licorice ultrafine ...

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Abstract

The invention discloses a composite culture medium for lactobacillus ferment and preparing method thereof. The culture medium contains 8-70% of licorice root filtered juice. In the culture medium, adding 7-25 g polypeptone in per 1000 ml licorice root filtered juice, also can add 2.0-5.0 g liver infusion. The selected 3 kinds of lactobacillus growing well in the culture medium has better effect than traditional lactobacillus selected culture medium-MRS culture medium. The formula of this culture medium is simple and needs simple preparing method, culture condition is easily controlled, low cost, safety and harmless, can be used widely for researching, developing and producing one of the most important probiotics-lactobacillus.

Description

technical field [0001] The invention relates to the technical field of production of medicines, food and food additives, veterinary medicine, biological feed and feed additives, in particular to a compound medium for lactobacillus fermentation and a preparation method thereof. Background technique [0002] Lactobacillus (Lactobacillus) is an important physiological bacteria and an important member of the normal flora in the intestinal tract of humans and other mammals. Since the physiological regulation of Lactobacillus and its fermentation products has been confirmed by long-term application practice and subsequent scientific research, the application range of Lactobacillus and its products in the fields of pharmaceuticals, food, veterinary medicine and feed production is constantly expanding, becoming these An important production strain in the industrial field; in recent years, due to its probiotic characteristics, structural characteristics and genetic characteristics, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225
Inventor 吴力克张娟胡锦华
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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