Cultural reduplication of excellent Chinese fir by fissue clone

Abstract

An asexual tissue culture method for reproduction of China fir includes such steps as induced culturing to obtain the bud of fir, inoculating it in the reproductive culture medium prepared from MS (1-1 / 2), 6-BA (0.5-1.0 mg / L) and NAA (0.2 mg / L), and culturing at 23+ / -2 deg.C under illuminance of 1600-2200 Lx. Its advantages are short period (35-45 days), high reproductivity and high survival rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically refers to a method for tissue culture and multiplication of fine Chinese fir clones. Background technique [0002] Chinese fir (Cunninghamia lanceolata (lamb.) Hook) is a species of economic forest with wide adaptability and excellent material quality. However, because Chinese fir is a cross-pollinated tree species, it is propagated by seeds, and the individual differentiation is large, so it is difficult to maintain the traits of the Chinese fir parent body; and conventional asexual reproduction methods such as cutting, layering or grafting are slow in breeding and difficult to meet the growing market demand. The tissue culture method is a rapid vegetative propagation technique. Selecting excellent Chinese fir clone materials and adopting the tissue culture method can not only maintain the excellent traits of the mother body, but also effectively save production costs. Although the...

AI Technical Summary

Problems solved by technology

Although the research on the tissue culture of Chinese fir tree species has been going on at home and abroad, there is no breakthrough in the proliferation technology in tissue culture. The existing Chinese fir tissue culture method has a long proliferation cycle and a low multiplication rate, so it has not been able to realize industrialized seedling production.